Table II

Assignments of the variants for histone H2A contained in the first and second HPLC peak

MassΔmAssignmentb
DeconvolutedaCalculated
Da
First HPLC peak
    13,421.813,421.5+0.3H2AZ
    13,900.213,899.2+1.0H2AQ
    14,006.814,006.3+0.5H2AO
    14,046.914,046.3+0.6H2AA
    14,046.914,048.3−1.4H2AO + 1 Acc
    14,086.914,086.2+0.7H2AO + 1Pd
Second HPLC peak
    13,817.113,817.1+0.0Q96KK5
    13,847.113,847.1+0.0H2AE
    13,928.013,927.1+1.0H2AE + 1 P
    14,002.214,002.3−0.1H2AC
    14,017.014,018.3−1.3H2AG
    14,017.014,016.3+0.7H2AL
    14,044.714,046.3−1.6H2AA
    14,044.714,044.3+0.4H2AC + 1 Ac
    14,081.514,082.3−0.7H2AC + 1 P
  • a The molecular masses of the histones were determined after deconvolution of the multiply charged ion series using an automated program.

  • b The major protein variants were assigned. The other variants may be present at lower levels.

  • c Acetylation is represented by Ac.

  • d Phosphorylation is represented by P.