Table II

Major human hair proteins identified in the insoluble fractions in all three individuals

At least four unique peptide sequences were identified from each individual. Only one protein entry is listed if several proteins were indistinguishable. The list is sorted in decreasing order of the sum of unique peptides in all three samples (not shown). IPI, International Protein Index.

IPI accession numberNumber of unique peptidesDescription
SMJYHR
IPI00013933 7211958Splice isoform 1 of desmoplakin
IPI00300053 297264Keratin, type II cuticular HB2
IPI00291540 443746Keratin, type I cuticular HA2
IPI00305719 394124Selenium-binding protein 1
IPI00376274 302528Predicted: similar to hypothetical protein A030011M19 (Ig-like domaina)
IPI00071509 213918Splice isoform 1 of plakophilin 1
IPI00420079 252920JUP (plakoglobin)
IPI00182655 302617Keratin, type II cuticular HB6
IPI00428691 282615Desmoglein 4
IPI00013890 17242014-3-3 protein σ
IPI00003935 etc.311214H2A family (O/Q, X, Y, Z)b
IPI00216711 22259Keratin-associated protein 11-1
IPI00032541 142020Keratin, type II cuticular HB5
IPI00465330 162315Type I hair keratin KA35
IPI00294649 121719Keratin, type I cuticular HA5
IPI00021439 191612Actin, cytoplasmic 1
IPI00397845 131023Similar to RIKEN cDNA 2310002B14
IPI00292715 131912Type I hair keratin 4
IPI00261031 131811Predicted: similar to hephaestin
IPI00303476 171410ATP synthase β chain, mitochondrial precursor
IPI00186711 111713Plectin 6
IPI00008524 102010Splice isoform 1 of polyadenylate-binding protein 1
IPI00020101 etc.131214H2B family (A, Q)c
IPI00455315 13167Annexin A2 isoform 2
IPI00152871 16127Leucine-rich repeat-containing protein 15 precursor
IPI00453473 14129HIST1H4F protein
IPI00419237 71612LAP3 protein
IPI00016752 12814S100 calcium-binding protein A3
IPI00013475 51018β-Tubulin
IPI00456625 111210Ka36 protein
IPI00025491 11129Eukaryotic initiation factor 4A-I
IPI00300376 10813Protein-glutamine glutamyltransferase E precursor
IPI00394683 81012Keratin-associated protein 10-12
IPI00219018 10811Glyceraldehyde-3-phosphate dehydrogenase
IPI00025447 10109Elongation factor 1-α 1
IPI00328398 71012Lysozyme g-like protein 2 precursor
IPI00291006 10118Malate dehydrogenase, mitochondrial precursor
IPI00019912 7157Peroxisomal multifunctional enzyme type 2
IPI00024067 7715Clathrin heavy chain 1
IPI00011229 9127Cathepsin D precursor
IPI00031423 9711Keratin, type I cuticular HA3-II
IPI00216984 999Calmodulin-like 3
IPI00220301 1089Peroxiredoxin 6
IPI00294840 7155Predicted: absent in melanoma 1 (crystalline β γa)
IPI00021405 9105Splice isoform 1 of lamin A/C
IPI00007702 887Heat shock-related 70-kDa protein 2
IPI00186290 6107Eukaryotic translation elongation factor 2
IPI00017292 5710Splice isoform 1 of β-catenin
IPI00022482 679Sialidase 2
IPI00013265 1084Keratin-associated protein 2
IPI00003865 569Splice isoform 1 of heat shock cognate 71-kDa protein
IPI00008486 569Protein-arginine deiminase type III
IPI00180675 848Tubulin α-3 chaind
IPI00003362 47878-kDa glucose-regulated protein precursor
IPI00465028 577Triose-phosphate isomerase 1
IPI00465248 666α-Enolase (IPI database version 3.05)
IPI00375323 756Keratin-associated protein 12-2
IPI00394674 864Keratin-associated protein KAP10-11
IPI00219575 558Bleomycin hydrolase
IPI00016461 5761-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase δ 1
IPI00472082 485Maspin precursor
IPI00465431 476LGALS3 protein
IPI00219757 566Glutathione S-transferase P
IPI00008529 85460 S acidic ribosomal protein P2
IPI00006663 575Aldehyde dehydrogenase, mitochondrial precursor
IPI00031461 457Rab GDP dissociation inhibitor β
IPI00000875 456Eukaryotic translation elongation factor 1 γ
IPI00414676 544Heat shock 90-kDa protein 1, β
IPI00385244 544Phosphoglycerate mutase 1 (brain)
IPI00479186 544Pyruvate kinase 3 isoform 1
  • a ProSite predictions of protein families and domains for uncharacterized proteins translated from genomic or cDNA sequences (us.expasy.org/prosite/).

  • b Histone 2A families O (or Q), X, Y, and Z (IPI00216457, IPI00059366, IPI00219037, and IPI00249267) are highly homologous and taken as a single entry. Otherwise with only one or two unique peptides, they would not be included as major proteins.

  • c Histone 2B families A and Q (IPI00020101 and IPI00003935) are highly homologous, sharing 10–12 peptides, and hence are combined as a single entry.

  • d IPI00387144 (α-tubulin, ubiquitous), found only in sample HR with a single unique peptide, is highly homologous to tubulin α-3, sharing six peptides. The peptides from this protein have been treated as if derived from tubulin α-3.