Table I

Interaction and expression data for potential UBX partners

Activation-deficient UBX mutants alone did not interact with the B42 activation domain in the yeast two-hybrid screen (7, 24). Interaction of BRD and YEMα with LEXA and LEXA-Lamin C indicates that these proteins are false positives. A percent retention comparable to EXD (76%) in phage display experiment independently verifies the protein interactions in a manner that controls for phage capacity and UBX activity. False positives are shown in bold text. Because UBX protein is produced in all tissue layers and throughout development, no partners could be eliminated by expression analysis. Expression data were extracted from FlyBase (www.flybase.org). EF2B, elongation factor 2B; NRT, Neurotactin; P120CTN, adherens junction protein p120; RPL22, ribosomal protein L22; RPN6, protease p44.5 subunit; RPS13, ribosomal protein S13; TRN, tartan; ZF30C, zinc finger protein 30C.

ProteinGene accession numberInteraction withPhage displaybExpression overlapcNuclear localizationa,d
LEXAaLEXA-Lamin CTissueStage
%
14-3-3ε U84897 NN72CNS, M, EctEC
AL L08401 NN70IDE, LY
ALY AF172637 NNN/AN/AN/AY
APT AF050379 NN72CNSE, LY
ARM X54468 NN65CNS, ID, M, EctE, LC
BRD U13067 Y Y 93
CBP80 AJ238970 NN65N/AN/AY
CYCK AF060516 NN76N/AN/AY
DIP1e NM167772 NN79CNS, IDE, LY
DSH L26974 NN80CNS, IDE, LN
EF1γ AF148813 NN80UUN
EF2B AE003781 NN80UUN
GLYP AF073179 NN 0
Hairy X15905 NN72EctEY
HSC70-4 L01500 NN78UUY
DMI-2 AF119716 NN77CNSEY
Motor protein AF145623 NNN/AN/AN/AU
NMO AI124322 NNN/AIDLU
NOC L14009 NN74CNS, EctEY
NRT AF132188 NNN/AM, IDE, L, PN
OTU X13693 NN71N/AN/AN
P120CTN AF220496 NNN/AN/AN/AN
RAD23 AF132147 NN74UUY
RPL22 U42578 NN81UUC
RPN6 AF160939 NNN/AN/AN/AY
RPS13 X91853 NN82UUC
SMOX AF078529 NN77CNS, M, IDE, LC
TFIIEβ U72893 NN54UUY
TRN I02078 NNN/ACNS, M, IDE, L, PN
ZF30C AF035275 NNN/AN/AN/AY
ZN72D U73125 NNN/AN/AN/AY
YEMα X63503 Y Y 76
  • a Y = yes; N = no.

  • b N/A, not available.

  • c CNS, central nervous system; Ect, ectoderm, ID, imaginal disc; M, mesoderm, U, ubiquitous; E, embryo; L, larva; P, pupa.

  • d C, conditional.

  • e Reported previously (24).