Table I

Results of the 1-D MS/MS analysis of cross-linked Skp1HBH protein complexes

All protein identities were validated manually. V-ATPase, vacuolar ATPase; ER, endoplasmic reticulum.

ORFNameComments
YDR328CSkp1
YOR133WEft1aElongation factor 2
YLR058CShm2Serine hydroxymethyltransferase
YJR033CRav1aRegulator of V-ATPase
YAL005CSsa1Heat shock protein
YLL024CSsa2Heat shock protein
YER103WSsa4Heat shock protein
YKL081WTef4Elongation factor 1-γ
YML088WUfo1aF-box protein
YDR502CSam2S-Adenosylmethionine synthetase
YLL050CCof1bCofilin
YKL152CGpm1Phosphoglycerate mutase
YDL132WCdc53aCullin
YNL311CSkp2aF-box protein
YKR059WTif1Initiation factor 4A
YBR196CPgi1Glucose-6-phosphate isomerase
YER062C/YIL053WGpp1/Gpp2cdl-glycerol-3-phosphatase 2
YIL148WRpl40A60 S ribosomal protein L40-A
YER177W/YDR099WBmh1/Bmh2c14-3-3 protein
YGR234WYhb1Flavohemoglobin
YKL156W/YHR021CRps27A/Rps27Bc40 S ribosomal protein S27-A
YNL121CTom70Translocase mitochondrial
YJR123WRps540 S ribosomal protein S5
YLR167WRps3140 S ribosomal protein S37
YMR186WHsc82Molecular chaperone
YBL030CPet9Mitochondrial ADP/ATP carrier
YJR027WTy1BTransposon Ty1 protein B
YMR046CTy1ATransposon Ty1 protein A
YMR056C/YBR085WAac1/Aac3cMitochondrial ADP/ATP carrier
YBR208CDur1,2bUrea amidolyase
YJL034WKar2ATPase, protein import into the ER
YFL009WCdc4aF-box protein
YGR155WCys4Cystathionine β-synthase
YPR181CSec23GTPase-activating protein
YKL006W/YHL001WRpl14A/Rpl14Bc60 S ribosomal protein L14-A
YBL072CRps8A40 S ribosomal protein S8
YDR037WKrs1bLysyl-tRNA synthetase, cytoplasmic
YKR014CYpt52GTP-binding protein
YLR293C/YOR185CGsp1/Gsp2cGTP-binding nuclear protein
YJL204CRcy1aF-box protein
YBL076CIls1bIsoleucyl-tRNA synthetase
  • a Proteins shown to interact with Skp1 (34, 40).

  • b Proteins with connections to the ubiquitin/proteasome pathway (40).

  • c Peptide sequence corresponding to either of these two proteins was identified.