Table I

List of proteins from human CRC tissue resolved on pH 7–10 2-DE gels and identified by MALDI-TOF/TOF MS

SNARE, soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptors; ALB, albumin; cds, coding sequence.

Spot no.Protein nameIPI_Human accession no.Molecular masspINo. of matched peaksProtein scoreIon scoreSequence coverageHighest ranking non-homologous protein score
Da %
1β-Globin gene from a thalassemia patient, complete cdsIPI0038295019,2046.28113672746550
2Phosphatidylethanolamine-binding proteinIPI0021944621,0277.4291691284040
3ALB proteinIPI0021677346,4425.7771161021352
4Peptidyl-prolyl cis-trans isomerase AIPI0041958518,0987.82885414257
5β-Globin gene from a thalassemia patient, complete cdsIPI0038295019,2046.2883742944640
6β-Globin gene from a thalassemia patient, complete cdsIPI0038295019,2046.2883132504651
7Peptidyl-prolyl cis-trans isomerase AIPI0041958518,0987.821223216645100
8Cofilin, non-muscle isoformIPI0001201125,5438.51134513585539
928-kDa Golgi SNARE proteinIPI0002944729,0629.36868681641
10Hemoglobin δ chainIPI0047301116,0287.97488462844
11Profilin-1IPI0021669115,0858.487132982842
12Peroxiredoxin 1IPI0000087422,3248.27112841775649
13Galectin-3IPI0046543126,0988.6162412072842
14Flavin reductaseIPI0021991022,7517.12484552150
15Peroxiredoxin 1IPI0000087422,3248.27796652845
16Mn-Superoxide dismutase, mitochondrial precursorIPI0002231424,8788.3541881562249
17Mn-Superoxide dismutase, mitochondrial precursorIPI0002231424,8788.35693662543
18Triosephosphate isomeraseIPI0064369626,9376.45132181105744
19Splice isoform 1 of heat shock cognate 71-kDa proteinIPI0000386571,0825.37224664052458
20Heat shock 70-kDa protein 1AIPI0051437770,2805.4874303651551
21Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.5831401311236
22Annexin A2IPI0045531538,6777.5683442602340
23Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.5831291141238
24Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.5836243842
25Phosphodiesterase 5A isoform 3IPI0006654394,9965.749561742
26α enolaseIPI0046524847,3506.99103112533645
27α enolaseIPI0046524847,3506.99103142333252
28β-Globin gene from a thalassemia patient, complete cdsIPI0038295019,2046.2842232002939
29Glyceraldehyde-3-phosphate dehydrogenaseIPI0055228237,0868.0815858435
30Heterogeneous nuclear ribonucleoprotein H1 (HNRPH1)IPI0002623049,5175.8961261071632
31Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.58475631245
3258-kDa protein (pyruvate kinase 3)IPI0038323758,3147.58143803043649
33Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.58591611743
34Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.5853773552348
35Fructose-bisphosphate aldolase CIPI0041826239,6996.4641251111543
36Transketolase variantIPI0064497068,5477.98153962039
37Voltage-dependent anion-selective channel protein 1IPI0021630830,7378.6392902254044
38Phosphoglycerate kinase 1IPI0016938344,8548.3102562093541
39Phosphoglycerate kinase 1IPI0016938344,8548.3103813113742
Da %
40Phosphoglycerate kinase 1IPI0016938344,8548.35105831539
41Fructose-bisphosphate aldolase CIPI0041826239,6996.463110931241
42Fructose-bisphosphate aldolase AIPI0046543939,7208.39660161743
43Fructose-bisphosphate aldolase AIPI0046543939,7208.3911100651955
44α2 globin variantIPI0041071415,3288.72167561036
45Galectin-3IPI0046543126,0988.6172051521742
46Keratin type II cytoskeletal 1IPI0055662466,1988.1627066157
47ALB proteinIPI0021677346,4425.77757291636
48Transgelin (smooth muscle protein)IPI0021613822,5228.88222981607345
49Complement C3 precursorIPI00164623188,5866.02126344849
50Complement C3 precursorIPI00164623188,5866.0225853144
51CatalaseIPI0046543659,8166.95122111383266
52Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.58480641168
53Hypothetical proteinIPI0044898525,7906.14394772170
54Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.5835949852
55Keratin 8 variantIPI0041841153,7055.5261141011346
56Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 (HNRPA2B1)IPI0047752237,3329.088170882441
57Flavin reductaseIPI0021991022,7517.12289781737
5822-kDa proteinIPI0047853922,3168.3861421123640
59Creatine kinase, ubiquitous mitochondrial precursorIPI0002463847,4068.662121701841
6071-kDa proteinIPI0047929171,8338.419581842
6134-kDa proteinIPI0041257734,5988.8435534943
62GTP:AMP phosphotransferase, mitochondrialIPI0046525625,4199.166126743443
63Single-stranded DNA-binding protein mitochondrial precursorIPI0002974417,2499.5931551112644
64β actinIPI0055072442,0785.29382741338
65Malate dehydrogenase, mitochondrial precursorIPI0029100635,9658.92389731536
66Carbonyl reductase (NADPH) 1IPI0029538630,5108.55373481448
67Keratin 1IPI0055662466,1988.1646351940
68Neuropolypeptide h3 (prostatic binding protein)IPI0021944621,0277.42132411365950
69Heat shock 70-kDa protein 8 isoform 1 variantIPI0064318871,0835.2875744935
70Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.671881611677
71TransgelinIPI0021613822,5228.9121891212943
72Glyceraldehyde-3-phosphate dehydrogenase, liverIPI0021901836,0708.631601251239
7357-kDa protein (keratin 10)IPI0047990256,6995.1466271042
74Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,2028.6390641247
75Malate dehydrogenase, mitochondrialIPI0029100635,9658.982532241539
76Malate dehydrogenase, mitochondrialIPI0029100635,9658.9210794741
77Hypothetical protein FLJ39660IPI0017635634,5618.17622051
78Carbonic anhydrase IIIPI0021841429,2856.92115961146
79UDP-glucose 6-dehydrogenaseIPI0003142055,6746.7184763542557
80Phosphoenolpyruvate carboxykinase, mitochondrial precursorIPI0029438071,4477.647331743
81UTP-glucose-1-phosphate uridylyltransferase 2IPI0039567655,8137.71079341549
Da %
82UTP-glucose-1-phosphate uridylyltransferase 2IPI0039567655,8137.7468591752
83AconitaseIPI0038284465,9347.9615647248
84Mn-Superoxide dismutase, mitochondrial precursorIPI0002231424,8788.35173782815552
85Glyceraldehyde-3-phosphate dehydrogenase, liverIPI0021901836,0708.5811593049
86Peptidyl-prolyl cis-trans isomerase AIPI0041958518,0987.8218153696155
87HADH2 protein (hydroxyacyl-coenzyme A dehydrogenase, type II)IPI0001772627,1347.66127774745
88ATP synthase, H+-transporting mitochondrial F1 complex, α subunit, isoform aIPI0054980559,8869.0718125224151
89Keratin 1IPI0055662466,1988.1613682748
90Heat shock 70-kDa protein 8 isoform 1IPI0064318871,0835.2846152838
91Pyruvate kinase 3 isoform 1 variantIPI0047918658,3827.2855162136
92Predicted: hypothetical proteinIPI0045636234,6139.8312643441
93Peptidyl-prolyl cis-trans isomerase AIPI0041958518,0987.8211175776256
94Voltage-dependent anion channel 1 proteinIPI0021630830,7378.63242881036344
95Destrin (actin-depolymerizing factor)IPI0047301418,8198.11101571085448
96Cytovillin 2IPI0038428216,2949.3231451361448
97Proteasome subunit β type 1IPI0002501926,7008.2712945343
98Nucleoside-diphosphate kinase BIPI0002626017,4018.52585513448
99Peptidyl-prolyl cis-trans isomerase AIPI0041958518,0987.82690475047
10060-kDa heat shock protein, mitochondrial precursorIPI0047210261,3465.7973272153
101Aconitase 2IPI0055071786,2527.6212117471748
102Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.6487751252
103Glyceraldehyde-3-phosphate dehydrogenaseIPI0021901836,0708.6579481239