Table II

List of all 67 phosphopeptides identified from plasma membrane preparations of Arabidopsis seedlings starved and resupplied with sucrose in three independent experiments

In those cases in which the site of phosphorylation could be determined, it is noted by pS for phosphoserine, pT for phosphothreonine, or pY for phosphotyrosine. Ambiguities are indicated by small letters in parentheses. The peptides for which quantitative information was retrieved are shown in bold (displayed in figures) or in italics (not in figures), and the normalized intensity ratios of phosphopeptides to non-phosphopeptides are shown. For proteins marked with asterisks, only phosphopeptides were identified. Time-dependent responses are considered as significant if p < 0.05 as determined by one-way ANOVA. AGI, Arabidopsis Gene Identifier; AA, amino acid; GAP, GTPase-activating protein; BRCT, BRCA1 C terminus; oxM, oxidized methionine.

Accession (AGI code)DescriptionSequence with modifications0351030Mascot scorem/zChargeModifi-cationLeft AARight AARelative mass errorp (one-way ANOVA)
ppm
Sucrose responses
    At2g18960Plasma membrane proton ATPases (AHA1 and AHA2) pTLHGLQPK 0.00−0.66−0.02−0.260.0452973.3811pTArgGlu−122.520.21
    At2g18960Plasma membrane proton ATPase (AHA1) GLDIDTAGHHYpTV 0.000.930.141.722.8853739.6721pTLysa−171.490.01
    At4g30190Plasma membrane proton ATPase (AHA2) GLDIETPSHYpTV 0.000.261.04−0.111.4658706.1021pTLys−257.390.03
    At5g57110Ca2+-transporting ATPase ACA8 pSEHADSDSDTFYIPSK 0.00−0.38−0.88−0.14−0.2944939.8521pSLysAsn−40.8730.04
    At5g62670Plasma membrane proton ATPase (AHA11)pTLHGLQAPDAK32616.0921pTArgMet148.469
    At1g52190Peptide transporter GFEKEDLpSPVVK 0.00−0.55−0.36−0.28−0.6160714.1521pSLysThr−232.970.24
    At1g59870Putative ABC transporterMpSNELAVPFDK26444.1331pSLysSer338.244
    At1g59870Putative ABC transporter SLpSTADGNR 0.00−0.41−0.2234500.5021pSTArgArg−276.86
    At1g61250Secretory carrier membrane protein (SC3) LpSPLPPEPVGFDYGR 0.000.18−0.300.00−0.0436862.6521pSArgThr280.1520.66
    At1g71880Sucrose transporter SUC1 DAAALETQpSPEDFDQPSPLR 0.00−0.41−0.46−0.290.40421133.7821pSLysLys−231.920.12
    At2g37170Aquaporin PIP2b SLGpSFR 0.000.230.360.23−0.1727373.6921pSLysSer118.6890.57
    At2g37170Aquaporin PIP2bSLGpSFRpSAANV24634.6022pSLys−173.62
    At2g39010Aquaporin PIP2e pSQLHELHA 0.00−0.621.190.60−1.7535507.5721pSArg−175.620.00
    At2g39010Aquaporin PIP2e TKDEL(t)EEE(s)L(s)GK 0.00−0.54−0.340.250.3575864.2222pSTMetAsp−188.40.11
    At2g39480Putative ABC transporter RQDpSFEMR * 30574.5921pSLysLeu−160.9
    At4g18910Major intrinsic protein-likeSGpSFLK * 33359.5921pSLysThr134.752
    At1g22280Protein phosphatase type 2C-like protein TDQAIL(s)N(ss)DLGR 0.00−0.340.02−0.17−0.7153779.1221pSLysGly370.1830.16
    At3g01490Protein kinase-like protein (s)L(s)DGEDNVNNTR * 0.00−0.81−0.421.280.6233750.5721pSLysHis−278.90.02
    At3g45780Nonphototropic hypocotyl 1 (NPH1) (ss)GEMSDGDVPGGR 0.00−0.080.360.520.1938715.5921pSArgSer−207.240.47
    At3g45780Nonphototropic hypocotyl 1 (NPH1)FLQG(s)G(t)DADELAK28726.1621pSTArgIle57.9872
    At4g19930F-box family proteinVPpSFIQR * 28463.5921pSArgLeu−152.46
    At4g35230Protein kinase family protein SYpSTNLAYTPPEYLR 0.000.170.240.740.5646927.5521pSLysAsn−405.110.40
    At4g35600Protein kinase family protein VGpSGMIVAIK * 0.00−0.040.74−0.020.2241527.9321pSArgArg117.7380.23
    At5g41260Protein kinase-like pSNPDVTGLDEEGR 0.00−0.14−0.950.33−0.1442735.1521pSArgGly303.7970.05
    At5g41260Protein kinase-like SYpSTNLAFTPPEYLR 0.000.950.840.360.5334919.6821pSLysThr−269.110.05
    AtCg00860ycf2.1 hypothetical proteinWYFELG(ts)MKK26517.133pSTLysPhe97.215
    At1g05150Putative O-GlcNAc-transferase DNDVPV(s)Y(s)GSGGPTK 0.000.640.890.990.1434830.6221pSArgSer333.230.21
ppm
    At1g19870Calmodulin-binding family protein KVpSNPSFIAAQSK 0.00−0.52−0.130.32−0.3540728.7021pSArgPhe−186.240.16
    At1g19870Calmodulin-binding family proteinVEPEE(s)E(s)DDVIIVR34898.1821pSLysLys−253.37
    At1g74690Calmodulin-binding family protein SGGMLETQNVGPEEIpSDDEI-ELPEGK * 0.00−0.080.210.660.5030952.0531pSArgSer303.1880.32
    At3g50840Phototropic-responsive NPH3 family proteinVIL(s)MK(ss)VLSPEIVER24983.8921pSArgSer185.175
    At5g37710Calmodulin-binding heat-shock protein like (s)N(s)GEFVLNDNVVPER 0.000.230.380.790.3955928.6721pSArg261.9060.56
    At5g45750Rab-type small GTP-binding protein-likeHSpTFENVETWLKELR24683.5231pTArgAsn115.35
    At5g61530Small G protein family protein/RhoGAPVGSLFKpSR50487.1421pSArgTrp58.8691
    At1g16120Wall-associated kinaseEVpSNELER28528.0821pSLysIle98.3942
    At1g53730LRR receptor-like protein kinase (SRF6) NKpSFDDEDSTR * 0.000.80−0.13−0.43−0.4754697.0921pSArgLys−211.80.04
    At3g02880, At5g53320, At5g16590, At4g23740, At3g08680, At5g05160, At3g17840, At5g58300, At1g48480, At2g26730Receptor-like kinase LRR III ApSAEVLGK 0.00−0.27−0.330.240.0550427.5621pSLysGly−152.150.39
    At3g02880Receptor-like kinase LRR III LIEEVSHSSG(s)PNPV(s)D 0.00−0.33−0.35−0.30−1.3737917.1421pSArg−285.80.01
    At3g14350LRR receptor-like protein kinase (SRF7) HKpSFDDDDSTMR 0.000.79−0.17−0.090.7564767.0821pSArgLys−242.190.03
    At5g10020Receptor-like kinase LRR III FSDQPVMLDVYpSPDR * 0.000.85−0.78−0.30−0.3259925.2721pSArgLeu391.2210.05
    At5g58300Receptor-like protein kinaseSPVQ(s)P(s)RDDMVDLPR29627.1431pSLysTrp130.953
    At1g11960Early responsive to dehydration protein-related(ss)PLHSGALVSK34631.6421pSArgPhe−198.18
    At3g28860ABC transporter NLSY(sy)STGADGR 0.00−0.75−0.62−0.52−0.2658735.8521pY 1pSArgIle105.750.12
    At1g53300Thioredoxin family protein(ssst)VATGETPIWK22772.1421pSTArgLys195.61
    At1g53300Thioredoxin family proteinIQI(t)GM(t)QSR24608.1021pTLysSer140.076
    At3g52400Syntaxin-like protein synt4 ANpSLVR * 0.00−0.23−0.38−0.7129370.0021pSArgSer−239.010.17
    At4g31480Coatomer β subunitAVNpYLLpSNVDK21466.1331pY 1pSArgVal55.4827
    At5g38190Myosin heavy chain-relatedMG(sss)PNNSVDSYIR24565.0531pSGlu316.743
    At2g20330Putative WD-40 repeat proteinIWDVNNFL(s)Q(t)QVIKPK * 24705.0531pSTArgLeu195.61
    At3g48740MTN3-like protein LGTV(ss)PEPISVVR 0.000.52−0.25−0.480.1246760.9321pSLysGln75.44270.18
    At1g22530SEC14 cytosolic factor family protein (y)GGL(s)KD(s)PF(t)VEDGVTE-AVVK 0.00−0.91−0.01−2.97−0.2347846.5031pY 2pSTLysSer−210.040.00
ppm
    At2g13940Putative retroelement pol polyproteinAQL(ss)CRQDGQAVIEYYGR28556.6741pSArgLeu−109.34
    At4g04430Putative transposon proteinGYNRpSMQVpYNWDR * 24617.6631pY 1pSLysSer88.4223
    At2g45820RemorinALAVVEKPIEEHpTPKK * 34623.4931pTLysAla−206.59
    At3g61260Remorin LpSFVR * 0.00−1.69−1.06−1.00−0.5425351.0421pSArgAla−113.250.00
    At5g24470Pseudo-response regulator 5 (APRR5)NEDGYSLpSVGK24417.1931pSLysIle49.1971
    At1g23870Glycosyltransferase family 20 protein (AtTPS9)KETYpSTAK26504.0821pSLysArg243.512
    At1g15400Unknown protein(tt)GRV(s)PAVDPP(s)PR24848.6121pTArgIle−301.2
    At1g15400Unknown proteinQG(ss)GIVFDDR * 36630.6621pSArgLeu102.587
    At1g15400Unknown protein VpSPAVDPPpSPR 0.00−0.72−1.00−0.120.0333641.1022pSArgIle−194.430.03
    At3g22390Unknown proteinKpSGGGAIGK35854.2311pSArgThr78.1825
    At3g25070RPM1-interacting protein 4 (RIN4) pSREESELK 0.00−0.70−0.51−0.7463507.5721pSArgGln65.4766
    At3g25070RPM1-interacting protein 4 (RIN4) ADEpSPEKVTVVPK * 0.00−1.14−0.85−0.24−1.2646739.7521pSArgPhe−113.180.01
    At4g02110BRCT domain-containing proteinVpSAPVTGNSNQK28641.0421pSLysGlu203.683
    At4g32285Epsin N-terminal homology (ENTH) domain-containing pSFGDVNEIGAR * 0.00−0.370.050.030.0858623.0721pSArgGlu261.7010.57
    At5g45510Leucine-rich repeat family protein KEDpTDGEDEIR * 0.00−0.82−1.02−0.39−0.1639693.6121pTLysSer−189.760.03
    At5g50101Unknown proteinAIQGTSTDPVIpTPLK * 34810.5221pTArgAsn−48.373
Mannitol responses
    At2g18960Plasma membrane proton ATPase (AHA1)GLDIDTAGHHYpTV0.000.991.231.421.5039739.8121pTLys−0.30650.02
    At2g18960Plasma membrane proton ATPases (AHA1 and AHA2)pTLHGLQPK0.00−0.06−0.10−0.26−0.2326487.2421pTArgGlu−0.69810.88
    At4g30190Plasma membrane proton ATPase (AHA2)GLDIETPSHYpTV0.000.130.22−0.070.2447706.3121pTLys2.774780.44
    At1g57990Purine permease-relatedQTTAEGSANPEPDQILpSPR*0.00−0.12−0.49−0.41−0.34581045.9721pSLysArg−0.46270.45
    At2g39010Aquaporin PIP2epSQLHELHA0.000.330.70−0.23−0.1318507.7221pSArg−2.66670.24
    At1g19870Calmodulin-binding family proteinVEPEEpSESDDVIIVR0.000.24−0.130.310.3447898.4021pSLysLys−0.16210.59
    At3g01830Calmodulin-related protein, putativeoxMLSLLGEpSKpSLK*0.000.440.360.6826741.3422pS, 1oxMArgAsp4.113990.47
    At3g08510Phosphoinositide-specific phospholipase C (PLC2)EVPpSFIQR0.000.310.240.040.1028528.2421pSArgAsn−2.2980.49
    At4g19930F-box family proteinVPpSFIQR0.000.53−0.030.1830463.7221pSArgLeu−0.36950.08
    At3g02880Receptor-like kinase LRR IIIApSAEVLGK17427.7021pSArgGly−1.2259
    At3g02880Receptor-like kinase LRR IIILIEEVSHSSG(s)PNPV(s)D0.00−0.17−0.38−0.33−0.3127917.3921pSArg0.016910.28
    At5g37970S-Adenosyl-l-methionine:carboxyl methyltransferasepYVNYFIVLKR0.000.03−0.300.140.0522697.8621pYLysLys−3.61590.15
    At3g08710Thioredoxin family proteinVTSIIDSVPEpSPQRP0.000.000.05−0.1658852.9121pSLys−2.54420.65
    At3g50280Transferase family proteinELpSPTFKER*0.000.991.131.7922593.7721pSArgVal−1.06410.00
ppm
    At3g48740MTN3-like proteinLGTV(ss)PEPISVVR0.000.840.530.1744760.8821pSLysGln1.606730.03
    At3g61260Remorin family proteinIALEpSESPAK*0.000.550.811.3440562.7621pSLysVal−0.91260.01
    At3g61260Remorin family proteinLpSFVR0.00−0.60−0.21−0.080.1224351.1621pSArgAla−3.04810.04
    At1g21580Hydroxyproline-rich glycoprotein family proteinSLGpYAR0.00−2.36−1.7417373.6621pYArgLys−1.2458
    At1g62060Expressed proteinQVEEVSKEpTK0.00−0.360.140.3421628.7821pTArgLeu−10.7080.28
    At1g66480Expressed proteinLDoxMLMLpSR0.000.440.600.2025537.7421pS, 1oxMArgArg6.816520.48
    At3g20110Cytochrome P450 family proteinLREEIDpSVVGETR0.000.310.240.040.0123528.2431pSArgLeu−9.97370.35
    At4g32285Epsin N-terminal homology (ENTH)pSFGDVNEIGAR0.000.170.180.170.1931622.7621pSArgGlu1.11570.86
    At4g39530Pentatricopeptide (PPR) repeat-containing proteinASASLTSLGLpSK0.000.6817607.8021pSArgGln−2.6624
  • a — indicates that there is no further amino acid in the sequence, i.e. if the tryptic peptide was the C- or N-terminal peptide of that protein.