Table I

Yeast mitochondrial phosphopeptides identified in this study

Phosphorylated peptides from highly purified yeast mitochondria were identified as described under ″Experimental Procedures.″ 80 different phosphorylation sites within 48 different proteins were identified. Asterisks indicate N-terminal acetylation. Spectra are listed in Supplemental Table 1. GPI, glycosylphosphatidylinositol.

ORFProteinGeneIdentified phosphopeptideaPosition of phosphorylated amino acidb
YER178W Pyruvate dehydrogenase complex E1-α subunit PDA1 YGGHpSMSDPGTTYRSer-313
YMR056C Mitochondrial inner membrane ADP/ATP translocator AAC1 STpSQRQFNGLLDVYKSer-155/Thr-156/Ser-157c
YBL030C Major ADP/ATP carrier of the mitochondrial inner membrane PET9 ESNFLIDFLMGGVSAAVAKpTAApSPIERThr-39, Ser-42
YJR077C Phosphate transporter MIR1 LVpSQPQFANGLVGGFSRSer-145
SVpSAAPAIPQYSVSDYMKSer-4
SpTLGCPPTIEIGGGGHThr-297
YBL099W ATP synthase subunit α ATP1 RpSVHEPVQTGLKSer-178
GVpSDEANLNETGRSer-57
YJR121W ATP synthase subunit β ATP2 GIpSELGIYPAVDPLDSKSer-373
EpTGVINLEGESKThr-237
VLDpTGGPISVPVGRThr-112
YPL078C ATP synthase subunit 4 ATP4 IDpSVSQLQNVAETTKSer-144
YDR298C ATP synthase subunit 5 ATP5 NpSSIDAAFQSLQKSer-48
GpTVTSAEPLDPKThr-139
YPL271W ATP synthase subunit ε ATP15 NGpTAASEPTPITKThr-52
YDL004W ATP synthase subunit δ ATP16 RpSYAEAAAASSGLKSer-21
YPR020W ATP synthase subunit g ATP20 QpSLNFALKPTEVLSCLKSer-62
*MLpSRIQNYTSGLVSKSer-3
YLR038C Cytochrome c oxidase, subunit VIb COX12 GIFAGDINpSDSer-82
YGL187C Cytochrome c oxidase, subunit IV COX4 EGpTVPTDLDQETGLARThr-55
YPR191W Ubiquinol cytochrome c reductase core protein 2 QCR2 pSAEDQLYAITFRSer-141
YPL262W Mitochondrial and cytoplasmic fumarase (fumarate hydratase), converts l-malate to fumarate as part of the tricarboxylic acid cycle FUM1 ANEPRIHELLpTKThr-428
YDR148C Component of the mitochondrial α-ketoglutarate dehydrogenase complex KGD2 GLVpTPVVRNAESLSVLDIENEIVRThr-340
YOR142W α subunit of succinyl-CoA synthetase LSC1 SGpTLTYEAVQQTTKThr-186
pSGTLTYEAVQQTTKSer-184
YOR136W Isocitrate dehydrogenase, subunit 2 IDH2 pTGDLAGTATTSSFTEAVIKThr-349
YKL085W Malate dehydrogenase MDH1 FIpSEVENTDPTQERSer-177
YLR304C Aconitase 1 ACO1 pTIFTVTPGSEQIRThr-409
YBL015W Acetyl-CoA hydrolase ACH1 pSQVVSNNPEMIRSer-350
YBR120C Translational activator of COB mRNA CBP6 MVDEKFpTEESINEQIRThr-97
YML120C NADH-ubiquinone oxidoreductase (AMID homolog) NDI1 FASTRpSpTGVENSGAGPTSFKSer-27, Thr-28
YHR008C Manganese-superoxide dismutase SOD2 LpTNpTKLAGVQGSGWAFIVKThr-147, Thr-149
YDR036c Member of the enoyl-CoA hydratase or isomerase family (fatty acid metabolism) EHD3 LLTKpSPSSLQIALRThr-326/Ser-328c
YPL186c Unknown function UIP4 GLDNLpSEGNDNDNTRSer-205
ELpSPNFSQEQTENKSer-185
GNVTFPpSPKSer-140
YOR374W Mitochondrial aldehyde dehydrogenase ALD4 EMpSVDALQNYLQVKSer-500
pTAESTPLSALYVSKThr-216
YML128C Protein of unknown function MSC1 DpTVFDKWpSSDQLTNWLESHKThr-237, Ser-243
YPL231W Fatty-acid synthase α subunit FAS2 QDISSSpTRThr-567
YPR184W Glycogen-debranching enzyme GDB1 DQPLYpTVKThr-45
YMR212C Protein of unknown function EFR3 DNQISpTSDLLSDSQVRThr-565
YAR035W Outer carnitine acetyltransferase, mitochondrial YAT1 LFVKSLLDQDApSDATKSer-517
YKL187C Protein of unknown function YKL187C KGEIYTYGDGSAIpSADRSer-678
KGEIYTYGDGpSAIpSADRSer-675, Ser-678
YKL217W Pyruvate and lactate/H+ symporter, member of the major facilitator superfamily (MFS) JEN1 VYpSQDQGVEYEEDEEDKPNLSAASIKSer-81
YINQVEEYEADGLpSISDIVEQKSer-584
*SSpSITDEKSer-4
SSpSITDEKISGEQQQPAGRSer-4
MIDSNVpSKSer-606
SSpSITDEKIpSGEQQQPAGRSer-4, Ser-11
YCR010C Protein may function as an acetate permease, Fun34 motif ADY2 IYpTGGDNNEYIYIGRThr-47
YNR002C Protein possibly involved in ammonium export, Fun34 motif ATO2 ALDpSSEGEFISENNDQSRSer-21/Ser-22c
ALDpSSEGEFISENNDQSRSer-21, Ser-22
ALDpSpSEGEFIpSENNDQSRSer-21, Ser-22, Ser-28
HSQEpSICKSer-40
EQpSSGNTAFENPKSer-7
pSDREQSSGNTAFENPKSer-2
ALDpSpSEGEFISENNDQSRSer-21, Ser-22
YDR384C Protein possibly involved in ammonium export, Fun34 motif ATO3 TSSApSpSPQDLEKSer-6, Ser-7
TSpSASSPQDLEKSer-4
TSpSApSSPQDLEKSer-4, Ser-6
TpSSApSSPQDLEKSer-3, Ser-6
TSSApSSPQDLEKSer-6
YBR054W Protein paralog of Mrh1p, has similarity to heat shock protein Hsp30 YRO2 AQEEEEDVApTDpSEThr-341, Ser-343
KAQEEEEDVApTDSEThr-341
KAQEEEEDVApTDpSEThr-341, Ser-343
YDR033W Membrane protein related to Hsp30p MRH1 APVApSPRPAATPNLSKSer-289
PAApTPNLSKThr-295
YGR086C Phosphorylation inhibited by long chain bases PIL1 ALLELLDDSPVpTPGETRPAYDGYEASKThr-233
YOL109W Protein that appears to act as a negative regulator of the Pkc1p-Mpk1p cell integrity pathway ZEO1 LEETKEpSLQNKSer-25
NEApTPEAEQVKThr-49
EQAEApSIDNLKSer-40
YMR031C Protein of unknown function YMR031c NNpSITSATSKSer-130
YBL064C Mitochondrial thiol peroxidase PRX1 INpSDAPNFDADTTVGKSer-53
YBR078W GPI-anchored protein of unknown function ECM33 LQpSNGAIQGDSFVCKSer-339
YML072C Protein involved in calcium-dependent phospholipid binding TCB3 SPSNLNSTSVpTPRThr-1350
YJR045C Mitochondrial heat shock protein 70 SSC1 AQFEpTLTAPLVKThr-330
YLR259C Mitochondrial heat shock protein 60 HSP60 pTNEAAGDGTTSATVLGRThr-102
YNL055C Porin 1 POR1 pSAVLNTTFTQPFFTARGSer-109
  • a Phosphorylated amino acid is indicated by p (pS for phosphoserine or pT for phosphothreonine).

  • b Positions were determined from precursor protein sequences.

  • c Manual inspection of spectra revealed ambiguous phosphorylation site.