Table II

New candidates of mouse kidney peroxisomes identified by protein correlation profiling

N/A, no information available; Pex, peroxisomal.

NameGene nameIPI accession no.Information on functional domains, transmembrane domains (TMDs), and further commentsPTS1 predicted by PSORTAbundance by peptide scoringLocalization listed by UniProtLocalization by PCP
Zinc-binding alcohol dehydrogenase domain-containing protein 2a Zadh2 00221569 Zinc-dependent oxidoreductaseYes5N/APex
Acyl-coenzyme A dehydrogenase family, member 11ab Acad11 00330747 N-terminal half: aminoglycoside phosphotransferase domain; C-terminal half: acyl-CoA dehydrogenase domainYes54N/APex
2810439K08RIK proteina Tmem135 00112089 Integral membrane protein of 52 kDa with 5 predicted TMDs (referred to as PMP52 in this work)No5N/APex
ACBD5 proteinab Acbd5 00229804 N-terminal half: acyl-CoA binding protein domain; may function as a carrier of acyl-CoA estersNo8N/APex
ATPase family, AAA domain-containing protein 1 Atad1 00108410 Belongs to the AAA superfamily of ATPases; may perform chaperone-like functions to assist (dis)assembly and function of protein complexesNo3N/APex
Abhydrolase domain-containing protein 14B Abhd14b 00111876 N-terminal half: abhydrolase:lysophospholipase domainNo4N/APex
RIKEN cDNA 1810022C23N/A 00112190 78% similar to peroxisomal Δ 32-enoyl-CoA isomerase in Mus musculus; belongs to the enoyl-CoA hydratase/isomerase familyYes25N/APex
Multifunctional protein ADE2 Paics 00322096 N-terminal half: phosphoribosylaminoimidazole-succinocarboxamide synthase; C-terminal half: phosphoribosylaminoimidazole carboxylase; protein is involved in purine biosynthesisNo2CytoplasmicPex
Acyl-CoA thioesterase 1 Acot1 00115871 N-terminal half: acyl-CoA thioester hydrolase and bile acid-CoA: amino acid N-acetyltransferase (BAAT); C-terminal half: dienelactone hydrolase; fulfills distinct functions in lipid metabolism; hydrolyzes bile acid-CoA esters in peroxisomes; induced in liver via proliferator-activated receptorsNo4CytoplasmicPex
Acyl-CoA thioester hydrolase 12 Acot12 00119810 N-terminal half: 2× brown fat-inducible thioesterase (BFIT) and brain acyl-CoA hydrolase (BACH); C-terminal half: steroidogenic acute regulatory-related lipid transfer (START) domain; involved in lipid transport and metabolismNo18CytoplasmicPex
Malate dehydrogenase 1b Mdh1 00336324 Member of the NAD-dependent 2-hydroxycarboxylate dehydrogenase family; involved in energy production and conversionNo3CytoplasmicDual
Fatty aldehyde dehydrogenase variant formbc Aldh3a2 00111235 NAD-dependent aldehyde dehydrogenase domain; catalyzes the oxidation of fatty aldehydes to fatty acids; mutations in the Aldh3a2 gene cause the early childhood-onset disorder Sjögren-Larsson syndrome (SLS)No9MicrosomalPex
Cytochrome b5bc Cyb5A 00230113 N-terminal half: cytochrome b5-like heme/steroid-binding domain; involved in electron transport and fatty acid metabolism; may be membrane-anchoredNo4MicrosomalDual
NADH-cytochrome b5 reductase homologbc Dia1 00110885 N-terminal half: oxidoreductase FAD-binding domain; C-terminal half: oxidoreductase NAD-binding domain; involved in lipid metabolismNo5MicrosomalDual
MOCO sulfurase C-terminal domain-containing 2a Mosc2 00123276 Fe-S protein with 1 predicted TMD that contains a MOSC N-terminal β barrel domain and was found to be localized in the inner mitochondrial (60)No5MitochondrialDual
  • a Localization of proteins in peroxisomes was confirmed by fluorescence microscopy.

  • b Protein identified previously in rat liver peroxisomes by Kikuchi et al. (11).

  • c Protein identified previously in rat liver peroxisomes by Islinger et al. (12).