Advertisement

November 2006

Volume 5Issue 11p2019-2202
Open Access
On the cover, two different chimeric proteins are expressed for the differential cytolocalization assay. The prey construct (A) encodes a protein of interest fused to a green fluorescent protein (GFP) reporter. The bait construct (B) consists of a second protein of interest fused with a membrane tether localization signal (D). When bait and prey are expressed together, an interaction will result in a change in the subcellular localization of the GFP-fused bait (C). Prot, protein. For details, see the article by Blanchard et al., pages 2175–2184....
On the cover, two different chimeric proteins are expressed for the differential cytolocalization assay. The prey construct (A) encodes a protein of interest fused to a green fluorescent protein (GFP) reporter. The bait construct (B) consists of a second protein of interest fused with a membrane tether localization signal (D). When bait and prey are expressed together, an interaction will result in a change in the subcellular localization of the GFP-fused bait (C). Prot, protein. For details, see the article by Blanchard et al., pages 2175–2184.

Research

Technology

Information

Advertisement