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November 2006

Volume 5Issue 11p2019-2202

Open Access

On the cover, two different chimeric proteins are expressed for the differential cytolocalization assay. The prey construct (A) encodes a protein of interest fused to a green fluorescent protein (GFP) reporter. The bait construct (B) consists of a second protein of interest fused with a membrane tether localization signal (D). When bait and prey are expressed together, an interaction will result in a change in the subcellular localization of the GFP-fused bait (C). Prot, protein. For details, see the article by Blanchard et al., pages 2175–2184....Show more

On the cover, two different chimeric proteins are expressed for the differential cytolocalization assay. The prey construct (A) encodes a protein of interest fused to a green fluorescent protein (GFP) reporter. The bait construct (B) consists of a second protein of interest fused with a membrane tether localization signal (D). When bait and prey are expressed together, an interaction will result in a change in the subcellular localization of the GFP-fused bait (C). Prot, protein. For details, see the article by Blanchard et al., pages 2175–2184.

November 2006

Editorial Board

Author Index

Research

A Quantitative Results-driven Approach to Analyzing Multisite Protein Phosphorylation

The Phosphate-dependent Phosphorylation Profile of the Transcription Factor Pho4 * S

Protein Profiling of Rat Ventral Prostate following Chronic Finasteride Administration

Identification and Localization of a Novel Putative Androgen-regulated Protein * S

Large Scale Identification and Quantitative Profiling of Phosphoproteins Expressed during Seed Filling in Oilseed Rape*S

Quantitative Proteomics Analysis of Detergent-resistant Membranes from Chemical Synapses

Evidence for Cholesterol as Spatial Organizer of Synaptic Vesicle Cycling * S

Modulation of Testicular Receptor 4 Activity by Mitogen-activated Protein Kinase-mediated Phosphorylation*

A Chemical Proteomics Approach for the Identification of Accessible Antigens Expressed in Human Kidney Cancer*S

Identification of PSME3 as a Novel Serum Tumor Marker for Colorectal Cancer by Combining Two-dimensional Polyacrylamide Gel Electrophoresis with a Strictly Mass Spectrometry-based Approach for Data Analysis*

Disease State Differentiation and Identification of Tuberculosis Biomarkers via Native Antigen Array Profiling*S

Identification and Characterization of New Protein Chemoattractants in the Frog Skin Secretome*

Peptide Microarray Analysis of Substrate Specificity of the Transmembrane Ser/Thr Kinase KPI-2 Reveals Reactivity with Cystic Fibrosis Transmembrane Conductance Regulator and Phosphorylase*S

LC-MS/MS Analysis of Apical and Basolateral Plasma Membranes of Rat Renal Collecting Duct Cells*S

Unusual N-Glycan Structures in α-Mannosidase II/IIx Double Null Embryos Identified by a Systematic Glycomics Approach Based on Two-dimensional LC Mapping and Matrix-dependent Selective Fragmentation Method in MALDI-TOF/TOF Mass Spectrometry*S

Identification of Apolipoprotein A-I as a “STOP” Signal for Myopia*

Technology

Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry*S

A Differential Cytolocalization Assay for Analysis of Macromolecular Assemblies in the Eukaryotic Cytoplasm*S

Differential Analysis of Saccharomyces cerevisiae Mitochondria by Free Flow Electrophoresis*S

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