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May 2019

Volume 18Issue 5p818-1035
Open Access
On the cover: Phagosomes are essential organelles linking innate and adaptive immunity during host defense that were analyzed by quantitative LC-MS/MS to characterize their spatiotemporal changes in dendritic cells (lower left). Displayed is a dendritic cell (upper left) phagocytosing antigen-coupled beads (red) that interact with lysosomes (green), while the actin cytoskeleton is shown in gray. The antigen-coupled beads, labeled with a red dye (Alexa 647), appear yellowish in this image because they have fused with surrounding lysosomes, which were labeled by a primary antibody (LAMP-1) and a secondary antibody carrying a green fluorophore (Alexa 488). A hallmark of the process of phagosome maturation, which we have characterized in our article, is the interaction of phagosomes (red in this case, because they carry the labeled antigen beads) with lysosomes (marked with a green dye in this case). When both proteins/dyes co-localize, you observe those yellow areas, while other parts that do not co-localize remain either in green or in red. An artistic view of the same cell (right), drawn and colored manually emphasizes the complexity of phagosome maturation. Image: Anne-Marie Pauwels, Illustration: Jasmien Vercruysse. For details see the article by Anne-Marie Pauwels et al., pages 909–922....
On the cover: Phagosomes are essential organelles linking innate and adaptive immunity during host defense that were analyzed by quantitative LC-MS/MS to characterize their spatiotemporal changes in dendritic cells (lower left). Displayed is a dendritic cell (upper left) phagocytosing antigen-coupled beads (red) that interact with lysosomes (green), while the actin cytoskeleton is shown in gray. The antigen-coupled beads, labeled with a red dye (Alexa 647), appear yellowish in this image because they have fused with surrounding lysosomes, which were labeled by a primary antibody (LAMP-1) and a secondary antibody carrying a green fluorophore (Alexa 488). A hallmark of the process of phagosome maturation, which we have characterized in our article, is the interaction of phagosomes (red in this case, because they carry the labeled antigen beads) with lysosomes (marked with a green dye in this case). When both proteins/dyes co-localize, you observe those yellow areas, while other parts that do not co-localize remain either in green or in red. An artistic view of the same cell (right), drawn and colored manually emphasizes the complexity of phagosome maturation. Image: Anne-Marie Pauwels, Illustration: Jasmien Vercruysse. For details see the article by Anne-Marie Pauwels et al., pages 909–922.

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