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February 2016

Volume 15Issue 2p341-751
Open Access
On the cover: On the cover: Müller cells (brownish), the highly arborized multi-functional macroglia of the retina (back right), are in intimate contact to all other retinal types such as cone (dark blue) and rod (bright blue) photoreceptors, bipolar (red), horizontal (dark green), amacrine (bright green) and ganglion cells (yellow). Their isolation with optimal conservation of fine side branches is challenging. To enable comprehensive proteomic profiling of Müller cells, which make up only 1.5% of the whole retinal cell population, gentle enzymatic digestion was applied in combination with magnetic activated cell separation. Label-free quantitative liquid chromatography-mass spectrometry comparing highly enriched Müller glia (>95%) to neuronal cells allowed identification of novel Müller cell-specific proteins such as peroxiredoxin 6 (purple), shown in the magnifying glass. Artwork by Jens Grosche (Effigos AG). For more details, see the article by Antje Grosche et al., pages 462–480....
On the cover: On the cover: Müller cells (brownish), the highly arborized multi-functional macroglia of the retina (back right), are in intimate contact to all other retinal types such as cone (dark blue) and rod (bright blue) photoreceptors, bipolar (red), horizontal (dark green), amacrine (bright green) and ganglion cells (yellow). Their isolation with optimal conservation of fine side branches is challenging. To enable comprehensive proteomic profiling of Müller cells, which make up only 1.5% of the whole retinal cell population, gentle enzymatic digestion was applied in combination with magnetic activated cell separation. Label-free quantitative liquid chromatography-mass spectrometry comparing highly enriched Müller glia (>95%) to neuronal cells allowed identification of novel Müller cell-specific proteins such as peroxiredoxin 6 (purple), shown in the magnifying glass. Artwork by Jens Grosche (Effigos AG). For more details, see the article by Antje Grosche et al., pages 462–480.

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