November 2013
Volume 12Issue 11p3006-3442
Open Access
On the cover: Stable-seq is a method that combines a simple genetic selection with
high-throughput DNA sequencing to assess the in vivo stability of a large number of variants of a protein. Each variant is fused to a
metabolic enzyme necessary in the selection condition, leading to a growth rate dependent
on the stability of the variant. DNA sequencing of variants before and after selection
enables the stability of thousands of variants to be scored simultaneously. The scores
determined by Stable-seq of variants carrying single mutations are visualized in this
heat map, with cell growth rates that would correspond to scores shown in the inset
boxes. For more details, see the article by Ikjin Kim et al., pages 3370–3378....Show more
On the cover: Stable-seq is a method that combines a simple genetic selection with
high-throughput DNA sequencing to assess the in vivo stability of a large number of variants of a protein. Each variant is fused to a
metabolic enzyme necessary in the selection condition, leading to a growth rate dependent
on the stability of the variant. DNA sequencing of variants before and after selection
enables the stability of thousands of variants to be scored simultaneously. The scores
determined by Stable-seq of variants carrying single mutations are visualized in this
heat map, with cell growth rates that would correspond to scores shown in the inset
boxes. For more details, see the article by Ikjin Kim et al., pages 3370–3378.
