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November 2013

Volume 12Issue 11p3006-3442
Open Access
On the cover: Stable-seq is a method that combines a simple genetic selection with high-throughput DNA sequencing to assess the in vivo stability of a large number of variants of a protein. Each variant is fused to a metabolic enzyme necessary in the selection condition, leading to a growth rate dependent on the stability of the variant. DNA sequencing of variants before and after selection enables the stability of thousands of variants to be scored simultaneously. The scores determined by Stable-seq of variants carrying single mutations are visualized in this heat map, with cell growth rates that would correspond to scores shown in the inset boxes. For more details, see the article by Ikjin Kim et al., pages 3370–3378....
On the cover: Stable-seq is a method that combines a simple genetic selection with high-throughput DNA sequencing to assess the in vivo stability of a large number of variants of a protein. Each variant is fused to a metabolic enzyme necessary in the selection condition, leading to a growth rate dependent on the stability of the variant. DNA sequencing of variants before and after selection enables the stability of thousands of variants to be scored simultaneously. The scores determined by Stable-seq of variants carrying single mutations are visualized in this heat map, with cell growth rates that would correspond to scores shown in the inset boxes. For more details, see the article by Ikjin Kim et al., pages 3370–3378.

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