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Molecular & Cellular Proteomics

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Animal models*

  • Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis
    You have access
    Integrated Analysis of Transcriptomic, miRNA and Proteomic Changes of a Novel Hybrid Yellow Catfish Uncovers Key Roles for miRNAs in Heterosis
    Guosong Zhang, Jie Li, Jiajia Zhang, Xia Liang, Xinyu Zhang, Tao Wang and Shaowu Yin
    Molecular & Cellular Proteomics July 1, 2019, First published on May 15, 2019, 18 (7) 1437-1453; https://doi.org/10.1074/mcp.RA118.001297

    mRNA-seq, miRNA-seq, proteomes of P. fulvidraco, P. vachelli, the hybrid Huangyou-1 livers were characterized. The nonadditive, HEB and ELD pattern were readily identified in transcriptional, post-transcriptional, or protein levels. Several predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and PRM. Immune defense, metabolism, digestion and absorption, cell proliferation and development involved in the generation of the heterosis phenotype. The high parental genes/proteins coupled with low parental miRNAs of the offspring are inherited from the mother or father.

  • Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells
    You have access
    Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells
    Samuel A. Myers, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev and Steven A. Carr
    Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; https://doi.org/10.1074/mcp.RA118.001259

    A streamlined and readily accessible sample preparation protocol has been developed to enable TMT-based proteomic profiling of relatively low numbers of cells directly from a flow cytometer. These methods were applied to 12 freshly isolated immune cell types from mice to a depth of over 7000 quantified proteins. These data recapitulate many aspects of known immunology, nominate new cell type specific protein markers, and provide evidence for post-transcriptional regulation of gene expression across the immune system.

  • Open Access
    Quantitative Proteomics Identification of Seminal Fluid Proteins in Male Drosophila melanogaster
    Irem Sepil, Ben R. Hopkins, Rebecca Dean, Marie-Laëtitia Thézénas, Philip D. Charles, Rebecca Konietzny, Roman Fischer, Benedikt M. Kessler and Stuart Wigby
    Molecular & Cellular Proteomics March 15, 2019, First published on October 4, 2018, 18 (Supplement 1) S46-S58; https://doi.org/10.1074/mcp.RA118.000831

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