Bacteria
Quantitative Proteomics of the 2016 WHO Neisseria gonorrhoeae Reference Strains Surveys Vaccine Candidates and Antimicrobial Resistance DeterminantsWe present the first large-scale quantitative MS-based profiling of cell envelopes and cytoplasmic proteins derived from a diverse panel of WHO gonococcal reference strains possessing all existing AMR profiles and intended for quality assurance in laboratory investigations. We describe nine novel gonorrhea vaccine candidates and six potential global proteomic AMR markers. A reference proteomics databank for gonococcal vaccine and AMR research endeavors is provided, which enables microbiological, clinical, or epidemiological projects and enhances the utility of the WHO reference strains.
A New Tool to Reveal Bacterial Signaling Mechanisms in Antibiotic Treatment and ResistanceChanges in proteome and phosphoproteome have been determined in E. coli treated with the antibiotics colistin or ciprofloxacin and in a bona-fide mcr-1 positive colistin resistant strain. The results reveal extensive phosphorylation on bacterial proteins and motif specific phosphorylation changes during resistance development. Moreover, regulated sites show high evolutionary conservation, indicating an important biological role. Together, this indicates that phosphorylation mediated signaling could be used as a specific target for drug design.