Energy metabolism
HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IVAssembly factors play a critical role in the biogenesis of mitochondrial respiratory chain complexes, some of which are present in large structures known as supercomplexes. Various assembly factors have been proposed as required for supercomplex assembly, including HIGD2A. We used quantitative proteomics and gene editing to clarify the function of human HIGD2A, revealing it to be a classical assembly factor required for biogenesis of mitochondrial DNA encoded COX3, a subunit of complex IV.
Discovery of a Redox Thiol Switch: Implications for Cellular Energy MetabolismA mechanism for the cellular effects of the gas H2S, is the oxidative modification of protein cysteine residues. We developed a quantitative proteomics tool to profile protein S-persulfidation in cellular proteomes. We discovered a Redox Thiol Switch of S-glutathioinylation to S- persulfidation of proteins, among them enzymes in cellular energy metabolism. This work allows identification of redox regulation of cysteine residues of proteins in physiological and disease states and can assist design of therapeutics for diseases such cancer and diabetes.
Metabolic Cross-talk Between Human Bronchial Epithelial Cells and Internalized Staphylococcus aureus as a Driver for InfectionStaphylococcus aureus invades bronchial epithelial cells to reach underlying lung tissue and to escape from the human immune defenses or antibiotic therapy. The internalized pathogen achieves these objectives by differentiation into growing and dormant subpopulations. Here we tracked the dynamic interactions between internalized bacteria and their host over four days by quantitative proteomics. The results highlight metabolic cross-talk between host and pathogen as a key driver for mutual adaptation and the outcome of infection.
In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal JellyThe hitherto depth proteomes of the hypopharyngeal glands (HGs) across the adult life of two stocks of honeybees with low (ITBs) and high royal jelly production (RJBs) uncover the molecular landscapes of the gland ontogeny and activity to match with gland age-specific tasks. Pathways involved in protein and energy metabolism are induced in HGs of RJB nurse bees to enhance royal jelly (RJ) secretion relative to ITBs. Our finding gains a novel mechanistic insight of the augment RJ-output in RJBs.
Hepatic Mitochondrial Defects in a Nonalcoholic Fatty Liver Disease Mouse Model Are Associated with Increased Degradation of Oxidative Phosphorylation Subunits2H2O-metabolic labeling approach was used to assess hepatic mitochondrial proteome dynamics in a diet-induced mouse model of non-alcoholic fatty liver disease (NAFLD). A Western-diet (WD) stimulated degradation of oxidative phosphorylation subunits in NAFLD mice liver. These changes were associated with decreased mitochondria content, impaired activities of electron transport chain complexes and ATP synthesis. Increased mitophagy contributed to enhanced degradation of mitochondrial proteins.