Molecular evolution*

  • The Old and the New: Discovery Proteomics Identifies Putative Novel Seminal Fluid Proteins in Drosophila
    The Old and the New: Discovery Proteomics Identifies Putative Novel Seminal Fluid Proteins in Drosophila
    Timothy L. Karr, Helen Southern, Matthew A. Rosenow, Toni I. Gossmann and Rhonda R. Snook
    Molecular & Cellular Proteomics March 15, 2019, First published on February 13, 2019, 18 (Supplement 1) S23-S33; https://doi.org/10.1074/mcp.RA118.001098

    We used discovery, bottom up proteomics to provide the first in-depth accessory gland proteome in D. pseudoobscura,. Computational bioinformatics identified >500 proteins in the secretory pathway of which 163 were annotated as extracellular., and therefore candidate, seminal fluid proteins. We further compared molecular rates of evolution between intra- and extra-cellular proteins, showing a hierarchy of rapid evolution with putative seminal fluid proteins evolving more rapidly than other secreted proteins, and those proteins evolving more rapidly than intra-cellular proteins.