Ribosomes*
Complexome Profiling Reveals Association of PPR Proteins with Ribosomes in the Mitochondria of PlantsPlant mitochondrial protein expression is complex and routinely involves pentatricopeptide repeat proteins for processing of transcripts. Although the composition and structure of the mitoribosomes in other eukaryotic kingdoms of life is well established, plant mitochondria have so far eluded a detailed analysis. Using a complexome profiling approach, this study provides an early glimpse on the composition of plant mitochondrial ribosomes and sheds new light on the process of mitochondrial gene expression in plants. Mitochondrial subunits were found to possess several additional proteins carrying pentatricopeptide repeats potentially involved in RNA editing. Other proteins are potentially involved in the processing of nascent proteins.
In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal JellyThe hitherto depth proteomes of the hypopharyngeal glands (HGs) across the adult life of two stocks of honeybees with low (ITBs) and high royal jelly production (RJBs) uncover the molecular landscapes of the gland ontogeny and activity to match with gland age-specific tasks. Pathways involved in protein and energy metabolism are induced in HGs of RJB nurse bees to enhance royal jelly (RJ) secretion relative to ITBs. Our finding gains a novel mechanistic insight of the augment RJ-output in RJBs.