S-palmitoylation
- Stress-induced Changes in the S-palmitoylation and S-nitrosylation of Synaptic Proteins
To improve simultaneous S-palmitoylation and S-nitrosylation site profiling, we developed a novel mass spectrometry-based strategy Palmitoylation And Nitrosylation Interplay Monitoring (PANIMoni). Using this approach we evaluated dynamic, site-specific changes in two important cysteine modifications, S-PALM and S-NO, in a mouse model of chronic stress. High-throughput analysis provided valuable insight into chronic, stress-induced remodeling of the synaptic proteome. Based on the obtained data, we postulate that the mechanism underlying chronic stress involves mutual competition between S-PALM and S-NO for modification sites.