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Molecular & Cellular Proteomics

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Program

Program: Wednesday, October 8, 2003

Molecular & Cellular Proteomics September 1, 2003, First published on October 2, 2003, 2 (9) 569-605;

  • 1. Osamu Hayaishi Lecture

    08:00–08:40, Room 710

    08:00 1.1 Organizing the Cell Through Modular Protein Interactions

    Tony Pawson, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

  • 2. Kunio Yagi Lecture

    08:45–09:25, Room 710

    08:45 2.1 Spatiotemporal Regulation of Cell and Body Functions by Rho and Rho Effectors

    Shuh Narumiya, Kyoto University, Faculty of Medicine, Kyoto, Japan

  • 09:25 Coffee Break

  • 3. Concurrent session 1

    Homocysteine: Biochemistry, Genetics, and Pathology

    Chair: John T. Brosnan, Memorial University of Newfoundland, St. John’s, NFL, Canada

    Co-Chair: Rima Rozen, Montreal Children’s Hospital, Montreal, QC, Canada

    09:35–12:45, Room 518ABC

    09:35 3.1 The Control of Homocysteine Metabolism

    John T. Brosnan, Memorial University of Newfoundland, St. John’s, NFL, Canada

    3.2 Genetic Risk Factors for Hyperhomocyteinemia: Studies in Humans and Mice

    Rima Rozen, McGill University, Montreal, QC, Canada

    3.3 Vascular Biochemistry of Homocysteine: Implications for Cardiovascular Disease

    Don Jacobsen, Cleveland Clinic Foundation, Cleveland, OH, United States

    3.4 Homocysteine, Folic Acid and Neural Tube Defects

    Nick Greene, Institute of Child Health, University College London, London, United Kingdom

  • 4. Concurrent Session 2

    Biological Energy Transduction

    Chair: Bridgette Barry, Georgia Institute of Technology, Atlanta, GA, United States

    Co-Chair: Bruce Charles Hill, Queen’s University, Kingston, ON, Canada

    09:35–11:05, Room 524ABC

    09:35 4.1 Molecular Architecture of Succinate Dehydrogenase (Complex ii) and Reactive Oxygen Species Generation

    Bernadette Byrne, Imperial College of Science, London, United Kingdom

    4.2 Light Reactions in Oxygenic Photosynthesis: Structure, Function, and Dynamics

    Bridgette Barry, University of Minnesota, St. Paul, MN, United States

    4.3 Cofactor Knockout Strategy Disentangles Cooperative Oxidative and Reductive Events in Cytochrome bc1; Elementary Nature of Energy Conversion and Regulation

    Les Dutton, University of Pennsylvania School of Medicine, Philadelphia, PA, United States

  • 5. Concurrent Session 3

    Antibody Engineering and Phage Display, Catalytic Antibodies, and Recombinant Antibody Technology

    Chair: Franck Perez, Institut Curie, Paris, France

    Co-Chair: Jean Gariépy, University Of Toronto, Toronto, ON, Canada

    09:35–11:05, Room 519AB

    09:35 5.1 The Recombinant Antibody Approach in Cell Biology: ‘‘Immunization’’ with Sub-cellular Compartments and Use of scFvs as Protein Conformation Sensors in Living Cells

    Franck Perez, Institut Curie, Paris, France

    5.2 The Development of Therapeutic Approaches Targeting IGE and MIGE

    Tse-Wen Chang, National Tsing Hua University, Hsinchu, Taiwan

    5.3 Fully Human Anti-cancer Immunoagents

    Claudia De Lorenzo, University of Naples “Federico II”, Napoli, Italy

  • 6. Concurrent Session 4

    Plant Signal Transduction I: Protein Kinases and Phosphoprotein Phosphatases

    Chair: Carol MacKintosh, University of Dundee, Dundee, Scotland, United Kingdom

    Co-Chair: Greg Moorhead, University Of Calgary, Calgary, AB, Canada

    09:35–11:05, Room 520CF

    09:35 6.1 Highly Conserved Protein Kinases Regulating Carbon and Amino Acid Metabolism

    Nigel Halford, Rothamsted Research, Harpenden, United Kingdom

    6.2 Signalling Pathways that Target Diverse Plant and Human 14-3-3-binding Partners

    Carol MacKintosh, University of Dundee, Dundee, Scotland, United Kingdom

    6.3 Phosphorylation of Metabolic Enzymes: Effects on Activity, Localization and Degradation

    Steven Huber, USDA/ARS and North Carolina State University, Raleigh, NC, United States

  • 7. Concurrent Session 5

    Amino Acid Metabolism in Health and Disease

    Chair: Philip Newsholme, University College Dublin, Dublin, Ireland

    Co-Chair: Margaret Brosnan, Memorial University, St. John’s, NFL, Canada

    09:35–11:05, Room 520BE

    09:35 7.1 Amino Acids, Insulin Secretion and Type-2 Diabetes

    Philip Newsholme, University College Dublin, Dublin, Ireland

    7.2 Aromatic Amino Acid Catabolism in Trypanosomes

    Juan José Cazzulo, Universidad Nacional de General San Martin, San Martin, Argentina

    7.3 Role of Glutamine Metabolism in Neutrophil Function

    Rui Curi, University of SÃo Paulo, SÃo Paulo, Brazil

  • 8. Concurrent Session 6

    Proteomics Cell Biology

    Chair: Kathryn Howell, University of Colorado Health Science Center, Denver, CO, United States

    09:35–11:05, Room 520AD

    09.35 8.1 Towards a Golgi Proteome

    Kathryn Howell, University of Colorado Health Science Center, Denver, CO, United States

    8.2 The Phagosome Proteome: New Paradigms in Cellular Immunology

    Michel Desjardins, Université De Montréal, Montréal, QC, Canada

    8.3 Proteomic Tools for Directing Cellular Process

    Brian Chait, United States

  • 9. Concurrent Session 7

    Biological Importance of Protein Phosphatases

    Chair: Shirish Shenolikar, Duke University, Durham, NC, United States

    Co-Chair: Marie Audette, Laval University Medical Center, Québec, QC, Canada

    11:15–15:15, Room 519AB

    11:15 9.1 Defining the Protein Phosphatase Complex that Regulates Cell Stress and Apoptosis

    Shirish Shenolikar, Duke University, Durham, NC, United States

    9.2 Targeting and Regulation of Protein Phosphatase 1: Role in Insulin Action

    Patricia T. W. Cohen, University of Dundee, Dundee, United Kingdom

    9.3 The EMBO Young Investigator Lecture: Protein Phosphatases Are Molecular Constraints on Learning and Memory

    Isabelle Mansuy, Swiss Federal Institute of Technology, Zurich, Switzerland

    9.4 Protein Tyrosine Phosphatases as Targets for Human Disease Treatments: From Diabetes and Obesity to Neuronal Regeneration and Cancer

    Michel Tremblay, McGill University, Montréal, QC, Canada

  • 10. Concurrent Session 8

    RNA Interference

    This session was made possible by an unrestricted educational grant by The Institute Of Genetics/CIHR.

    Chair: Patrick Provost, Laval University, Quebec, QC, Canada

    Co-Chair: Claude Lazure, Montreal Clinical Research Institute, Montréal, QC, Canada

    11:15–12:45, Room 524ABC

    11:15 10.1 Genome-wide RNAi Screens in Drosophila Cells

    Amy Kiger, Harvard Medical School, Boston, MA, United States

    10.2 Dicer and the RNA Interference (RNAi) Pathway

    Patrick Provost, Laval University, Quebec, QC, Canada

    10.3 Crystal Structure and Binding Specificity of an RNA Silencing Suppressor

    Traci Hall, National Institutes of Health, Research Triangle Park, NC, United States

  • 11. Concurrent Session 9

    Plant Signal Transduction II: Plant Defense Responses

    Chair: Jacques-Henry Weil, Université Louis Pasteur, Strasbourg, France

    Co-Chair: Caron Helbing, University of Victoria, Victoria, BC, Canada

    11:15–12:45, Room 520CF

    11:15 11.1 The Role of Antioxidant-mediated Signal Transduction During Stress

    Christine Foyer, IACR Rothamsted Research, Harpenden, United Kingdom

    11.2 Long Distance Signaling in Systemic Acquired Resistance

    Huub Linthorst, Leiden University, Leiden, The Netherlands

    11.3 Phosphite Blocks Phosphate Sensing in Plants and Yeast

    Bill Plaxton, Queen’s University, Kingston, ON, Canada

  • 12. Concurrent Session 10

    Applications of Mass Spectrometry to Analysis of the Proteome

    This session was made possible through an unrestricted educational grant by the Protein Engineering Network of Centres of Excellence—PENCE.

    Chair: Michael Moran, MDS Proteomics Inc., Toronto, ON, Canada

    Co-Chair: John Wilkins, Manitoba Centre for Proteomics, University of Manitoba Winnipeg, MB, Canada

    11:15–12:45, Room 520BE

    12.1 Assignment of Post Translational Protein Modifications, an Essential Argument for Proteomics

    Peter Roepstorff, University of Southern Denmark, Odense, Denmark

    12.2 Comprehensive Proteome Analysis by Mass Spectrometry

    Liang Li, University of Alberta, Edmonton, AB, Canada

    12.3 Functional Proteomics Applications in Drug Discovery and Development

    Michael Moran, MDS Proteomics Inc., Toronto, ON, Canada

  • 13. Concurrent Session 11

    Proteomics, Mass Spectrometry, and Quantitation

    Chair: Alma L. Burlingame, University of California, San Francisco, CA, United States

    11:15–12:45, Room 520AD

    11:15 13.1 The Relative Merits of MALDI and ESI Tandem Mass Spectrometry Platforms in Proteomic Scale Protein Identification and Quantitation

    Alma L. Burlingame, University of California, San Francisco, CA, United States

    13.2 High Throughput Quantitative Proteomics: Progress and Challenges

    Ruedi Aebersold, Institute for Systems Biology, Seattle, WA, United States

    13.3 Adaptation, application and Comparison of Three Methods for Comparative Proteomics in Studies of Drug Resistance

    Catherine Fenselau, University of Maryland, Baltimore, MD, United States

  • 12:45 Lunch Break, Poster Viewing, & Exhibits

  • Industry-Sponsored Sessions: Invitrogen, Bruker Daltronics

  • 14. Oral Session 1

    Functional Proteomics/Protein Interactions Part I

    Co-Chair: Daniel Figeys, MDS Proteomics Inc., Toronto, ON, Canada

    Co-Chair: Mike Tyers, Toronto, ON, Canada

    14:45–16:15, Room 520CF

    14:45 14.1 Large-scale Analysis of the Phosphoproteome

    Daniel Figeys, MDS Proteomics Inc., Toronto, ON, Canada

    15:00 14.2 Study of the Phosphoproteome in Human Cell Lines

    Michel Caron, Ufr Smbh, Université Paris 13, Bobigny Cedex, France

    15:00 14.3 Functional Proteomics of Blood Monocytes

    Haifeng Wu, Ohio State University, Columbus, OH, United States

    15:30 14.4 Dynamic Changes of the Protein Composition of the Spliceosome as Revealed by a Proteomic Approach

    Henning Urlaub, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany

    15:45 14.5 An Integrated Strategy to Identify New Regulators of Protein Transport

    Jeremy Simpson, EMBL, Heidelberg, Germany

    16:00 14.6 Novel Specificity of the SH3 Domain Explored by Peptide Arrays, Oriented Peptide Libraries and Structural Biology

    Shawn Li, University of Western Ontario, London, ON, Canada

  • 15. Oral Session 2

    Technical Innovation—Mass Spectrometry

    Chair: Pierre Thibault, Caprion Pharmaceuticals, Montréal, QC, Canada

    14:45–16:15, Room 524ABC

    14:45 15.1 Proteome Analysis of Human Immunodeficiency Virus Type 1 Infection of CD4+-T-Cell Lines

    Deborah Diamond, University of Washington, Seattle, WA, United States

    14:57 15.2 A New Mass Tagging Chemistry for Proteomics

    Gordon Nicol, Agilent Technologies, Wilmington, DE, United States

    15:09 15.3 De Novo Sequencing of Tryptic Peptides Using a Novel CD Based Chemical Derivatisation and a MALDI-QIT-TOF MS

    Chris Sutton, Shimadzu Biotech, Manchester, United Kingdom

    15:21 15.4 A MALDI-TOF Mass Spectrometer with Orthogonal Injection

    Joseph DiCesare, PerkinElmer Sciex Instruments, Shelton, CT, United States

    15:33 15.5 Characterisation of Complex Protein Samples Using LC-MALDI QIT TOF MS

    Rachael Martin, Shimadzu Biotech, Manchester, United Kingdom

    15:45 15.6 T3-Sequencing, a Novel Top-Down Method for the Direct Characterization of the Termini of Intact Proteins

    Detlev Suckau, Bruker Daltonics, Bremen, Germany

    15:57 15.7 Enhancement of Sensitivity and Sample Throughput in Proteomics Analyses Using Targerted LC-MS-MS Analyses

    Pierre Thibault, Caprion Pharmaceuticals Inc., Montréal, QC, Canada

  • 16. Oral Session 3

    Medical Proteomics—Neuronal Diseases

    Chair: Claudio Cuello, McGill University, Montréal, QC, Canada

    14:45–16:15, Room 518ABC

    14:45 16.1 Proteomics of Brain Proteins in a Parkinson’s Mouse Model

    Dijana Sagi, University Clinic Charité, Berlin, Germany

    15:03 16.2 Amyloid b Protein-induced Neuronal Toxicity Investigated by Differential Proteomics DIGE and ICAT

    Cécile Cren-Olivé, Université des Sciences et Technologies, Villeneuve D’Ascq, France

    15:21 16.3 Proteome Analysis of Cerebrospinal Fluids

    Chris Turck, Max Planck Institute of Psychiatry, Munich, Germany

    15:39 16.4 2-D Gel-based and ICAT-based Proteomic Analyses of Rat and Human Brain Endothelial Cells Exposed to In Vitro Ischemia

    Arsalan Haqqani, Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada

    15:57 16.5 Methamphetamine-induced Behavioral Sensitization Is Associated with ERK2 Downregulation

    Jean Lud Cadet, National Institute on Drug Abuse/National Institute of Health, Baltimore, MD, United States

  • 17. Oral Session 4

    Proteomes of Plants

    Chair: Mike Deyholos, University of Alberta, Edmonton, AB, Canada

    14:45–16:15, Room 520BE

    14:45 17.1 Proteomic Analysis of Soybean Proteins Implicated in Food Quality and Safety

    Steve Gleddie, Agriculture and Agrifood Canada, Ottawa, ON, Canada

    15:00 17.2 Method Development for Proteome Analysis of Arabidopsis Seeds Produced by Different Ecotypes (Accessions) and by Transgenic Events

    Klaus-dieter Jany, Federal Research Centre for Nutrition, Karlsruhe, Germany

    15:15 17.3 A Comparison of Herbivore Response Mechanisms in a Variety of Plant Species

    Lippert Dustin, University of British Columbia, Vancouver, BC, Canada

    15:30 17.4 Proteomic Analysis of the Protein Profiles Expressed During the Developmental Stages in Rice Seed Maturation

    Young Mok Park, Korea Basic Science Institute, Daejeon, South Korea

    15:45 17.5 Pedigree Analysis of Rice Seeds Using Proteomic Approaches

    Jingqiang Wang, Beijing Genomics Institute, Beijing, P.R. China

    16:00 17.6 A Highly Glycosylated Peroxidase in Post-harvested Cassava (Manihot Esculenta Crantz) Tubers

    Tipaporn Limpaseni, Chulalongkorn University, Bangkok, Thailand

  • 18. Oral Session 5

    Capillary Separation Method

    Chair: Arthur M Moseley, Proteomic Technologies, Genomic and Proteomic Sciences, Genetics Research, GlaxoSmithKline, NC, United States

    14:45–16:15, Room 520AD

    14:45 18.1 Sensitivity Enhancement in Nano-LC-nano-ESI-MS/MS Using Capillary Column of 50 μm Inner Diameter: Application to Human Cancer Cell Differential Proteomics

    Cécile Cren-Olivé, Université des Sciences et Technologies, Villeneuve D’ascq, France

    15:00 18.2 High Speed Proteome Analysis Using Monolithic Capillary LC Coupled to MALDI-QQTOF-MS

    Devanand Pinto, National Research Council, Halifax, NS, Canada

    15:15 18.3 Polymer-based Monolithic Capillary Columns for Proteomics Applications

    Séverine Le Gac, Université des Sciences et Technologies de Lille, Villeneuve D’Ascq, France

    15:30 18.4 Multi-dimensional Liquid Chromatography with Parallel Capillary Columns Followed by Mass Spectrometry for High Throughput Proteomic Studies

    Xiangmin Zhang, Fudan University, Shanghai, P.R. China

    15:45 18.5 Strategies for Optimizing LC/MS/MS Performance for Capillary and Nanoscale Separations of Complex Proteomic Samples: An Integrated System Approach

    Steven Cohen, Waters Corporation, Milford, MA, United States

    16:00 18.6 Glycotyping of Transferrin Isoforms in Human Malignant Neoplasia

    Franz Jacques Legros, Chu André Vésale, Montigny-le-Tilleul, Belgium

  • 19. Concurrent Session 12

    Educational Session: Mass Spectrometry

    Chair: Peter James, Wallenberg Laboratory II, Lund University, Lund, Sweden

    14:45–18:00, Room 514ABC

    14:45 19.1 Overview of MS Developments

    Robert J Cotter, Johns Hopkins University School of Medicine, Baltimore, MD, United States

    19.2 MS Database Searching

    David Fenyo, Amersham Biosciences, Piscataway, NJ, United States

    19.3 MS/MS Database Searching

    Jimmy Eng, Institute for Systems Biology, Seattle, WA, United States

    19.4 Manual MS/MS Interpretation

    Richard Johnson, Amgen Corporation, Seattle, WA, United States

  • 16:15 Coffee Break

  • 20. This session has moved to Friday

  • 21. Oral Session 6

    Functional Proteomics/Protein Interactions Part II

    Co-Chair: Daniel Figeys, MDS Proteomics Inc, Toronto, ON, Canada

    Co-Chair: Mike Tyers, Toronto, ON, Canada

    16:30–18:00, Room 520CF

    16:30 21.1 Integrative Genomics of Cell Size Control

    Mike Tyers, Toronto, ON, Canada

    16:48 21.2 Waling Down a Protein Interaction Map: A Study of the pp2a-Type Phosphatases in Mammalian Cells

    A.C. Gingras, United States

    17:06 21.3 The First Map of the Human Protein Module, the WW Domain, Points to New Signaling Steps, Pathways and Networks

    Marius Sudol, Mount Sinai School of Medicine, New York, NY, United States

    17:24 21.4 Regulatory Networks of the Human RNA Polymerase II Basal Transcription Machinery Resolved Using a Targeted Proteomics Approach

    Célia Jeronimo, IRCM, Montréal, QC, Canada

    17:42 21.5 Beyond Proteomics: Protein Interactomics

    Kurt Dejgaard, McGill University, Montréal, QC, Canada

  • 22. Oral Session 7

    Cell Signaling

    Chair: Jeffery Wrana, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada

    16:30–18:00, Room 518ABC

    16:30 22.1 A Biochemical Genomics Approach to the Identification of Substrates of the S. cerevisiae Ste20 Kinases, Ste20 and Cla4

    Robert Annan, McGill University, Montréal, QC, Canada

    16:42 22.2 Extracellular ATP Affects Osteblasts Biology by Acting at the Transcriptional Level

    Gianluca Tell, University of Trieste, Trieste, Italy

    16:54 22.3 Tracking Protein Kinase Signalling Pathways on Macroarrays with Antibodies and Peptide Antibody Mimetics (PAMs)

    Steven Pelech, Kinexus Bioinformatics Corporation, Vancouver, BC, Canada

    17:06 22.4 Integrated Functional-Molecular Analyses to Explore Models of Regulated Membrane Fusion

    Jens Coorssen, University of Calgary, Calgary, AB, Canada

    17:18 22.5 Endosomal Signaling Via the EGF Receptor

    Jisheng Liu, McGill University, Montréal, QC, Canada

    17:30 22.6 Proteome Profiling of Transforming Growth Factor-Beta Signalling

    Serhiy Souchelnytskyi, Ludwig Institute for Cancer Research, Uppsala, Sweden

    17:42 22.7 A Novel High Throughput Luminescence-based Assay to Analyze Dynamic Protein-Protein Interactions in Mammalian Cells

    Miriam Barrios-Rodiles, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada

  • 23. Oral Session 8

    HUPO Initiative—Antibodies

    Chair: Marius Ueffing, Institute of Human Genetics, Neuherberg, Germany

    Co-Chair: Stefan Dubel, Technical University of Braunschweig, Institute of Biochemistry & Biotechnology, Neuherberg, Germany

    16:30–18:00, Room 519AB

    16:30 23.1 A Human Proteome Resource Based on Affinity Proteomics

    Mathias Uhlen, Royal Institute of Technology (KTH), Stockholm, Sweden

    16:50 23.2 The European Proteome Initiative (EPI)

    Marius Ueffing, German Society for Proteome Research and Institute of Human Genetics, GSF, München, Germany

    17:10 23.3 A Proteomics-based Strategy to Establish Antibody Bank for Human Liver Proteomics

    Qi-hong Sun, Beijing Institute of Radiation Medicine, Beijing, P.R. China

    17:25 23.4 Recombinant Antibodies for Proteome Research

    Stefan Duebel, Technical University of Braunschweig, Braunschweig, Germany

    17:40 23.5 One-step Microarray Detection and Isotyping of Monoclonal Antibodies

    Federico De Masi, Embl, Heidelberg, Germany

    17:50 23.6 Generation and Characterization of Murine Monoclonal Antibodies Against Human Plasma Proteins Using Unknown and Native Multi-proteins as Immunogens

    Ming Li, The First Military Medical University, Guangzhou, P.R. China

  • 24. Oral Session 9

    Proteomes of Microbes

    Chair: Michael Ellison, University of Alberta, Edmonton, AB, Canada

    16:30–18:00, Room 520BE

    16:30 24.1 Genome-scale Tools, Resources and Data for E. coli Systems Analysis

    Hirotada Mori, Nara Institute of Science and Technology, Nara, Japan

    16:52 24.2 Systematic Identification of Protein Complexes in E. coli

    Jack Greenblatt, University of Toronto, Toronto, ON, Canada

    17:14 24.3 A Quantitative Correlation of E. coli Gene Expression with Protein Expression During Aerobic and Anaerobic Growth

    Joel H. Weiner, University of Alberta, Edmonton, AB, Canada

    17:36 24.4 Altered States: Adaptive Antibiotic Resistance and Swarm Cell Differentiation in Salmonella

    Michael Surette, University of Calgary, Calgary, AB, Canada

  • 25. Oral Session 10

    Structural Proteomics

    Chair: Mirek Cygler, Biotechnology Research Institute, NRC, Montréal, QC, Canada

    16:30–18:00, Room 524ABC

    16:30 25.1 Insight into Biochemical Processes of Escherichia coli Using Structural Proteomics

    Allan Matte, Biotechnology Research Institute, Montréal, QC, Canada

    16:48 25.2 Development of an Experimental Data Tracking Database for Structural Genomics Research

    Stéphane Raymond, Department of Biochemistry, McGill University, Montréal, QC, Canada

    17:06 25.3 Structural Reorganization of Proteins Revealed by Radiolysis and Mass Spectrometry: Divalent Cation Dependent Structure of Monomeric and Filamentous Actin

    Mark Chance, Albert Einstein College of Medicine, Bronx, NY, United States

    17:24 25.4 Probing Conformational Changes and Interactions of Proteins and their Micromolecular Assemblies Using Hydroxyl Radical Mediated Protein Footprinting

    Janna Kiselar, Albert Einstein College of Medicine, Bronx, NY, United States

    17:42 25.5 A New Method for the Proteomic Analysis of Membrane-bound N-glycosylated Proteins from Caenorhabditis elegans

    Xiaolian Fan, The Hospital for Sick Children, Toronto, Canada

  • 25A. HUPO Plenary Lecture

    This session was made possible through an unrestricted educational grant by McGill University.

    18:00–18:50, Room 710

    25A.1 Electrospray Wings for Molecular Elephants

    John Fenn

  • 26. Poster Session 1

    Cell Signaling

    26.1 Proteomics Approaches to Identify Phosphorylation Modifications Induced by Galectin-1 in Jurkat T-Cells

    Raymonde Joubert-Caron, Université Paris 13, Bobigny, France

    26.2 Elucidating Novel Cell Signalling Events in Response to Microtubule-interfering Agents Using KinetworksTM Analysis

    Hong Zhang, Kinexus Bioinformatics, Vancouver, BC, Canada

    26.3 Functional Characterization of Grp78 as the Alpha-2-Macroglobulin Signaling Receptor in Parameters of Signaling Pathways Activated Consequent to Agonist Binding

    Uma Misra, Duke University Medical Center, Durham, NC, United States

    26.4 A Comparative Study of the Effect of Nitric Oxide on Colony Forming Ability and Differentiation of Human Erythroid and Myelouid Leukemia Cell Lines

    Mina Rafiei, Institute of Biochemistry and Biophysics University of Tehran, Tehran, Iran

    26.5 Characterization of a BS69-related Transcriptional Regulator, BSR

    Anatoly Mikhailik, State University of New York at Stony Brook, Stony Brook, NY, United States

    26.6 Developed Method Application for Nitrite Ion (NOØ2) Analysis of Tib-186 Macrophage Like Cell Lines by Rapid Isocratic HPLC System with High Sensitive Glassy Carbon Electrochemical Detector

    Manuchehr Ghojaie, Institute of Biochemistry and Biophysics, Tehran, Iran

    26.7 Silencing of CREB Gene Expression Abolishes cAMP Induced Cellular Proliferation: Dependence on PI 3-Kinase Signaling Pathway

    Salvatore Pizzo, Duke University Medical Center, Durham, NC, United States

    26.8 Dissection of the Mechanisms of Survival, Growth and Proliferation of Immature and Mature B Cells

    Derek Blair, University of Glasgow, Glasgow, United Kingdom

    26.9 Characterization of Tristetraprolin as a Zinc-dependent mRNA ARE-binding Protein

    Heping Cao, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States

    26.10 Interaction of ARF Isoforms with Intracellular Loop 3 and Carboxy Tail Domains of the 5-HT2A Receptor

    Derek Robertson, Edinburgh University, Edinburgh, United Kingdom

    26.11 The AAA ATPase p97/VCP Is Involved in the Cellular Response to DNA Damage

    Martin Latterich, McGill University, Montréal, QC, Canada

    26.12 Casodex Treatment Induces Hypoxia-related Gene Expression in the LNCaP Prostate Cancer Progression Model

    Gopalakrishnan Velliyur, University of Nebraska Medical Center, Omaha, NE, United States

    26.13 A Dual Functional Role for the XLP Gene Product SAP/SH2D1A in Signaling Through the SLAM Family of Immune Receptors

    Shawn Li, University of Western Ontario, London, ON, Canada

    26.14 Differential ERK Signalling in Immature B Cells

    Catriona Ford, University of Glasgow, Glasgow, United Kingdom

    26.15 Exploring the Collagen-binding Domain of the DDR Tyrosine Kinase Receptors

    Wolfgang Vogel, University Of Toronto, Toronto, ON, Canada

    26.16 Regulation of RNA Polymerase III Transcription by Mammalian Target of Rapamycin (mTOR)

    Emma Graham, University of Glasgow, Glasgow, United Kingdom

    26.17 Insights into a Single Rod-like Helix in Activated Radixin Required for Membrane-Cytoskeletal Crosslinking

    Klaus Hoeflich, Ontario Cancer Institute and University of Toronto, ON, Canada

    26.18 The Drosophila TGF-beta Family Type II Receptor, Wishful Thinking Activated Multiple TGF-β Signaling Pathways

    Si Tuen Lee-hoeflich, University of Toronto, Toronto, ON, Canada

    26.19 The Role of Integrin-linked Kinase in Angiogenesis Through the Regulation of HIF-1a and VEGF Expression

    Clara Tan, University of British Columbia, Vancouver, BC, Canada

    26.20 Effects of MEK1 Inhibitor on Suppression of Invasiveness of High Metastatic Rat Prostatic Adenocarcinoma Cell Line, MLL

    Tuangporn Suthiphongchai, Mahidol University, Bangkok, Thailand

    26.21 Requirement of Phospholipase CD4 for Ca2+ Mobilization Essential for Acrosome Reaction in Sperm

    Kiyoko Fukami, Tokyo University of Pharmacy and Science, Hachioji, Tokyo, Japan

    26.22 Effect of Schistosomal Antibodies on Cell Proliferation of S. mansoni Schistosomula

    Mohamed Abdel Fattah, Ain Shams University, Cairo, Egypt

    26.23 Role of Ergosterol as a Signal Molecule of Fungal-pathogen Recognition

    Vladimir Mikes, Masaryk University, Brno, Czech Republic

    26.24 HBX Protein Up-regulates the Expression of hTERT and Its Activity

    Xiaodong Zhang, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin, P.R. China

    26.25 Cloning and Expression of the GTPase Activating Protein (GAP) for RhoA in Escherichia coli

    Anna-Maria Ochocka, Medical University of Gdansk, Gdansk, Poland

    26.26 A Phosphoprotein-Phosphatase Inhibitor Exhibits an Interferon-gamma Mimetic Activity

    Marie Audette, Laval University Medical Center, Québec, QC, Canada

    26.27 The Studies on Lg1–3 Module of Human Laminin 4

    Yujing Zhang, Agriculture and Animal Science College, Changchun, Jilin, P.R. China

    26.28 Inactivation of the GRB10 Gene Affects Embryo Size, Cytoskeletal Structures and Apoptosis

    Andre Nantel, National Research Council, Montréal, QC, Canada

    26.29 ICAM-1 Gene Transcription Stimulated by Phosphotyrosine Phosphatase Inhibitor bpV(Pic) Requires JAK-1, JAK-2 and p38 MAPK

    Isabelle Drolet, Laval University Medical Center, Québec, QC, Canada

    26.30 The Temporal Characteristics of cAMP Production in Response to Full and Weak Partial Agonists in CHO-K1 Cells Expressing the Human Beta-2-Adrenoceptor

    Stephen Hill, University of Nottingham, Nottingham, United Kingdom

    26.31 Time Course of Agonist-stimulated CRE-mediated Reporter Gene Transcription in CHO Cells

    Jillian Baker, University of Nottingham, Nottingham, United Kingdom

    26.32 Signal Transduction Via the Thromboxane A2 Receptor in Vascular Smooth Muscle

    David Wilson, University of Calgary, Calgary, AB, Canada

    26.33 Kinetics of Carboxypeptidase-D (CPD) and Its Nuclear Isoform (CPD-N) in Breast Cancer and Immune Tumor Cells

    Padraic O’ Malley, Dalhousie University, Halifax, NS, Canada

    26.34 Activation of Calcium Release by Calcium Current in Rat Cardiac Myocytes

    Ivan Zahradnik, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovakia

    26.35 Expression of p21WAF1/CIP1 Through Sp1 Sites by Histone Deacetylase Inhibitor Apicidin Requires PI 3-Kinase-PKCepsilon Signaling Pathway

    Hyang Woo Lee, Sunukyunkwan University, Suwon, South Korea

    26.36 Palmitoylation Regulates GDP/GTP Exchange of G Protein by Affecting the GTP Binding Activity of Goa

    Youguo Huang, Insitute of Biophysics, Chinese Academy of Science, Beijng, P.R. China

    26.37 The Effect of 25-CROW-5 and 18-CROWN-6 on Mouse Bone Marrow Hamatopoietic Cell Culture and Their Interactions with c-AMP, DNA and Histones

    Anahita Lashgari, Islamic Azad University, Science & Research Branch, Tehran, Iran

    26.38 The Serine/Threonine Phosphatase POPX and Its Regulation of Cell Signaling and Morphology

    Cheng-gee Koh, Institute of Molecular and Cell Biology, Singapore, Singapore

    26.39 p38 and JNK Inhibit Fas-mediated Caspase-8 Activation but Differentially Regulate Type II Apoptotic Signaling in Jurkat T Lymphocytes

    Leon Tourian, Jr., McGill University Health Centre, Montréal, QC, Canada

    26.40 A T Cell PTP Interacting Protein (TcPTPIP51) Is Expressed in Dependence of Differentiation

    Albrecht Stenzinger, Institute of Anatomy and Cell Biology, Giessen, Germany

    26.41 Purification of Yeast Recombinant Ssb1p/Hsp75 and Its Interaction with Calmodulin

    Vania Paschoalin, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    26.42 Nuclear Targeting of Alpha4 Phosphoprotein Is Not Due Entirely to O-GlcNAc Modification

    Shauna Dauphinee, Dalhousie University, Halifax, NS, Canada

    26.43 Mechanistic Link Between Intestinal Insulin Signaling and Lipoprotein Production

    Lisa Federico, The Hospital for Sick Children, Toronto, ON, Canada

    26.44 Regulation of N-Cadherin Expression by RhoA and Cdc42/Rac1 During Neurodetermination of P19 Stem Cells Involves ERK and p38 MAPK

    Isabel Laplante, Université du Québec a Montréal, Montréal, QC, Canada

    26.45 Cardiac Telokin Is Localized to the Intercalated Disc

    Aniko Rokolya, University of Calgary, Faculty of Medicine, Calgary, AB, Canada

    26.46 Role of DDR1 in Breast Cancer Cell Invasion

    Yun Huang, University of Toronto, Toronto, ON, Canada

    26.47 Proteomic Analysis of Protein Kinase Components of Steroid Hormone-mediated Signaling

    Paul Khan, Laval University Medical Research Center (CRCHUL), Québec, QC, Canada

    26.48 β-Catenin Signaling Facilitates Transendothelial Migration of Melanoma Cells

    Jianfei Qi, University of Toronto, Toronto, ON, Canada

    26.49 Haematopoietic Progenitor Kinase (HPK1) Is Constitutively Fragmented in Human Platelets

    Kenneth Wong, University of Calgary and Canadian Blood Services, Calgary, AB, Canada

    26.50 Dopamine D2 Receptor-induced ERK Translocation to the Nucleus Involves Multiple Pathways

    Patrick Jean Rogue, Université Loius Pasteur, Strasbourg, France

    26.51 Regulation of Calcium Signals in the Cell Nucleus

    Patrick Jean Rogue, Université Loius Pasteur, Strasbourg, France

    26.52 GIT1 Provides PAK Localization and Activation Cues

    Zhuoshen Zhao, Institute of Molecular and Cell Biology, Singapore, Singapore

    26.53 Rho GTPase, Tcl/Tc10betaL and RGS2 Promote the Adipocyte Differentiation in the Presence of PPARgamma Ligand

    Makoto Nishizuka, Nagoya City University, Grad. Sch. of Pharm. Sci., Nagoya, Japan

    26.54 Overexpression of Alpha1b-Adrenergic Receptors Alters the ERK Pathway

    Marie-Josée Benoit, Montreal Heart Institute, Montreal, Quebec, Canada

    26.55 Acute Changes in U937 Nuclear Ca2+ That Precede Programmed Cell Death Due to MK 886

    Ken Anderson, Rush Medical College, Chicago, IL, United States

    26.56 Role of MAP Kinase Signal Transduction Pathway in UVB Induced Activation of Murine Peritoneal Macrophges In Vitro

    Gautam Sethi, Banaras Hindu University, Varanasi, U.P., India

    26.57 Pathogenesis-related Proteins Mediated Host Resistance to Phytophagous Insects in Tomato Accessions

    Srinivasan Ramasamy, Tamil Nadu Agricultural University, Coimbatore, India

    26.58 Binding Characteristics of PTP-BL PDZ Domains

    Lieke Van Den Berk, University of Nijmegen, Nijmegen, Netherlands

    26.59 A Domain of Tyrosine Phosphorylation in the N-terminus Regulates the Functional Expression of GIRK5 Potassium Channels

    S. Ivonne Mora Herrera, Nacional Autonomous University of Mexico UNAM, México City, México

    26.60 Endosomal Signaling Via the EGF Receptor

    J. Liu, McGill University, Montreal, QC, Canada

  • 27. Poster Session 2

    Capillary Separation Method

    27.1 A Microfluidic Solution for Protein Qa/Qc

    Tanja Neumann, Agilent Technologies Deutschland GmbH, Waldbronn, Germany

    27.2 Application of Capillary Isoelectric Focusing with Laser-induced Fluorescence Detection to the Analysis of Myosin Regulatory Light Chain Phosphorylation

    Mitsuya Shiraishi, University of Calgary, Calgary, AB, Canada

    27.3 Novel Fluorescein Affinity Chromatography for Protein Characterization Using Mass Spectrometry

    Shu-Hui Chen, National Cheng Kung University, Tainan, Taiwan

    27.4 Dual-Gradient, 2-D Capillary LC/MS/MS for Complex Proteomics Samples

    Remco Van Soest, LC Packings/Dionex, Sunnyvale, CA, United States

  • 28. Poster Session 3

    HUPO Initiative—Antibodies

    28.1 Effect of Schistosomal Antibodies on Cell Proliferation of S. mansoni Schistosomula

    Yehia Shaker, Ain Shams University, Cairo, Egypt

    28.2 A Small Scale, High Throughput Method for M13 Phage Based Proteomics

    Wai-choi Leung, Tulane University School of Medicine, New Orleans, LA, United States

  • 29. Poster Session 4

    Proteomes of Microbes

    29.1 System Analysis of Helicobacter pylori Clinical Isolates

    Vadim Govorun, V. N. Orekhovich Institute of Biomedical Chemistry, Moscow, Russia

    29.2 Neurospora Cell Wall Proteome Analysis by Mass Spectrometry

    P. John Vierula, Carleton University, Ottawa, ON, Canada

    29.3 The Brucella Orfeome and Interactome Projects

    Xavier De Bolle, URBM, University of Namur, Namur, Belgium

    29.4 Thermostable Amylases from an Acidophilic Fungus Arachniotus sp.

    Muhammad Asghar, University of Agriculture, Faisalabad, Punjab, Pakistan

    29.5 Histone-like Proteins in Thermophile and Mesophile Bacteria

    Zahra Hagihassan, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    29.6 Genome-wide Analysis of Protein-Protein Interaction in Escherichia coli

    Maki Maeda, CREST JST, Tokyo, Japan; Research & Education, Centre for General Information, NAIST, Tokyo, Japan

    29.7 Proteomics of the Outer Membrane of Actinobacillus pleuropnumoniae

    Lorne I. Budman, McGill University, Montréal, QC, Canada

    29.8 De Novo Sequencing and Analysis of Post-translational Modifications in SARS Viral Proteins, by (Off-line HPLC)-MALDI-QqTOF Measurements

    Kenneth Standing, University of Manitoba, Winnipeg, MB, Canada; Manitoba Centre for Proteomics, Winnipeg, MB, Canada

    29.9 Comparative Proteomics of the Human Pathogen Campylobacter jejuni

    Tom Slyker, Bio-Rad Laboratories, Hercules, CA, United States

    29.10 Proteomic Analysis on Structural Proteins of SARS Coronavirus

    Wantao Ying, Beijing Institute of Radiation Medicine, Beijing, P.R. China

  • 30. Poster Session 5

    Structural Proteomics

    30.1 Structure and Functions of Human Ubiquitin-like Post-translational Modifier SUMO-1/2/3 Proteins

    Steven S. L. Li, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC

    30.2 Cloning, Production and Crystallization of Mammalian Proteins for Structural Genomics

    Michael Sacher, McGill University and Biotechnology Research Institute, Montréal, QC, Canada

    30.3 Alternative Protein Structure Determination Olga Tcherkasskaya, Georgetown University, Washington, VA, United States

  • 31. Poster Session 6

    Technical Innovation—Mass Spectrometry

    31.1 Simple Stable Isotope-containing Matrix-purifiable Labels for Proteomics

    Stephanie Trudel, Ste. Justine Hospital, Montréal, QC, Canada; University of Montréal, Montréal, QC, Canada

    31.2 Parallel Purification of Serum Peptides for Mass Spectrometry

    Elena Chernokalskaya, Millipore Corporation, Danvers, MA, United States

    31.3 Integrating a New Peptide De-novo Sequencing Tool for Sophisticated Data Analysis

    Ulrike Schweiger-Hufnagel, Bruker Daltonik, Bremen, Germany

    31.4 An Alternative Modifier of Cysteinyl Groups in 2D-Electrophoresis Permitting Identification by MALDI-TOF MS

    Jan Goscinski, Amersham Biosciences, Uppsala, Sweden

    31.5 Novel Stable-Isotope Labeling for Quantitative Proteomics and its Application for Protein Expression Profiling

    Shu-Hui Chen, National Cheng Kung University, Tainan, Taiwan

    31.6 A New Protein Chip Using for Electroblotting Accelerating Proteome Analysis

    Lyang-ja Lee, Mitsubishi Pharma Corporation, Hirakata, Osaka, Japan

    31.7 Integrated Proteomic Analysis: Reducing the Workload of Low-throughput Instruments

    Detlev Suckau, Bruker Daltonics, Bremen, Germany

    31.8 Analysis of Human Serum/Plasma Using Cleavable ICAT

    Kit-yi Leung, Institute of Psychiatry, London, United Kingdom

    31.9 Rapid and Reproducible Sample Complexity Reduction and Identification of Low-abundance Proteins Using Micro-Scale Mini-Columns and Orthogonal MALDI-TOF Mass Spectrometry

    Mary F. Lopez, PerkinElmer Life and Analytical Sciences, Boston, MA, United States

    31.10 Investigation of a Mammalian Cellular Model for Differential Protein Expression Analysis Using 1-D PAGE and Cleavable ICAT Reagents

    Tony Hunt, Applied Biosystems, Framingham, MA, United States

    31.11 Determination of Phosphorylation Sites in an Unknown Sample (ABRF PRG03)

    Oleg Krokhin, University of Manitoba, Winnipeg, MB, Canada and Manitoba Centre for Proteomics, Winnipeg, MB, Canada

    31.12 Fast Analysis of Complex Protein Mixtures by LC-MALDI-TOF/TOF-MS

    Detlev Suckau, Bruker Daltonics Inc., Billerica, MA, United States

    31.13 2D-Chromatography Using a Novel High Capacity Ion Trap for Faster Proteomics Applications

    Markus Lubeck, Bruker Daltonik GmbH, Bremen, Germany

    31.14 Combining LC Separation with Simultaneous Online-ESI and Offline-MALDI-MS/MS Analysis for High Sequence Coverage

    Markus Lubeck, Bruker Daltonik GmbH, Bremen, Germany

    31.15 Automated De Novo Sequencing of Proteins Using Isotopic Labeling and Tandem Mass Spectrometry

    Matthew Sniatynski, University of British Columbia, Vancouver, BC, Canada

    31.16 Proteome Profiling Using Isotopically Differentiated Protein Derivatization

    Ken Chisholm, National Research Council of Canada, Institute for Marine Biosciences, Halifax, NS, Canada

    31.17 Fully Automated Two-dimensional Nano-Electrospray LC/MS System for Low-attomol Proteomic Analysis

    Dirk Chelius, Thermo Electron, San Jose, CA, United States

    31.18 Dramatic Productivity Improvements for Protein Identification Using a New Two-dimensional Ion Trap Mass Spectrometer

    Dirk Chelius, Thermo Electron, San Jose, CA, United States

    31.19 Multiplex LC-MS System for the Rapid Identification of Glycoproteins

    Eric Bonneil, Caprion Pharmaceuticals Inc., Montréal, QC, Canada

    31.20 Identification of Sub-fmol Protein Mixtures Using AP MALDI-MS/MS Data from a Linear Ion Trap Mass Spectrometer

    Ken Miller, Thermo Electron Corporation, San Jose, CA, United States

    31.21 Strategy for Maximizing Protein Identification by MALDI-MS/MS Using a Linear Ion Trap Mass Spectrometer

    Ken Miller, Thermo Electron Corporation, San Jose, CA, United States

    31.22 Study and Troubleshooting of a Periodic Variation in the Total Ion Current in and LC-Q-TOF and Its Effect on Spectrum Quality

    Stephan Laperrière, Montréal Proteomics Network, Montréal, QC, Canada

    31.23 MALDI-QTOF Vs LC-QTOF: Analysis of 2-D Gel Spots

    Marcos Di Falco, Montréal Proteomics Network, Montréal, QC, Canada

  • 32. Poster Session 7

    Medical Proteomics—Neuronal Diseases

    32.1 Proteomic Analysis in Transient Occlusion of the Middle Cerebral Artery

    Young Ae Lee, Neurotech Pharmaceutical Corporation, Suwon, South Korea

    32.2 Display and Functional Proteomics of Neuronal Cell Apoptosis in Cortical Cell Cultures

    Lee Jae-keun, Ajou University, Suwon, South Korea

    32.3 Display and Functional Proteomics of NMDA-induced Neuronal Cell Death in Cortical Cell Cultures

    Kyoung Joon Moon, Ajou University, Suwon, South Korea

    32.4 Proteomic Analysis of Murine Cortical Cell Cultures Following Kainate Administration

    Lee Jin-hwan, Ajou University, School of Medicine, Suwon, South Korea

    32.5 Expression of Coxsakie-Adenovirus Receptor and Integrin Subunits b3 and b5 on the Surface of Human NT2 Neurons

    Deqi Huang, National Research Council of Canada, Ottawa, ON, Canada

    32.6 Age-Dependent Changes in Oxidative Stress Markers and Antioxidant Enzymes in the Brain of OXYS Rats

    Tatiana Shcheglova, Institute of Cytology and Genetics, Novosibirsk, Russia

    32.7 Proteomic Analysis of the Role of Alpha-B-Crystallin in Different Neurodegenerative Diseases

    Claus Zabel, University Clinic Charité, Berlin, Germany

    32.8 Protein Profiling of Cerebral Spinal Fluid for ALS Specific Biomarkers

    Robert Bowser, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States

    32.9 Proteomic Analysis of Human Cerebral Cortex in Epileptic Patients

    Yong-geun Kwak, Chonbuk National University Medical School, Chonju, South Korea

  • 33. Poster Session 8

    Protein-Protein Interactions

    33.3 Structural Determinants of Oligomerisation in the Salmon Serum C-type Lectin

    David Hudson, Dalhousie University, Halifax, NS, Canada

    33.4 Deciphering Protein Interactions Using Surface Enhanced Laser Desorption/Ionization (SELDI)

    Wang Zheng, Ciphergen Biosystems, Inc., Fremont, CA, United States

    33.5 Structural Analyses Suggest the Existence of Functionally Important Inter-domain Interactions in the Co-chaperone Murine Stress-inducible Protein 1

    Odutayo Odunuga, Rhodes University, Grahamstown, South Africa

    33.6 Polycystin-2 Attaches to Actin Cytoskeleton

    Qiang Li, University of Alberta, Edmonton, AB, Canada

    33.7 A Novel Import Pathway Promotes Nuclear Import of Heat Shock Protein 70 in Response to Ethanol Stress

    Xin Xin Quan, McGill University, Montréal, QC, Canada

    33.8 Effects of Site-directed Mutations on p26, a Small Heat Shock/Alpha-Crystallin Protein from Artemia franciscana

    Yu Sun, Dalhousie University, Halifax, NS, Canada

    33.9 Optimizing Experimental Design in High-throughput Interaction Proteomics

    Heilbut Adrian, University of Toronto, Toronto, ON, Canada

    33.10 Proteomic Analysis of Poly(ADP-Ribose), PARG and PARP Interactors

    Guy Poirier, Research Center Of CHUL, Ste-Foy, QC, Canada

    33.11 Photoaffinity Labeling of Proteins in Nuclear Extract by Base Excision Repair Intermediates

    Natalia Lebedeva, Institute of Bioorganic Chemistry, Novosibirsk, Russia; Institute Jacques Monod, Paris, France

    32.12 H-Ras Homolog Proteins Regulate p73β Function Through Protein-Protein Interactions in Nucleus

    Kynug-hee Choi, Chung-Ang University, Seoul, South Korea

    33.13 Novel Partner Proteins of Adenovirus Penton

    Jadwiga Chroboczek, Institut de Biologie Structurale, Grenoble, France

    33.14 Making Random Peptide Library with Genomic DNA

    Haiming Huang, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, P.R. China

    33.15 Spectroscopic Analysis of DmsD, a Twin-Arginine Binding Protein from E. coli

    Kwabena Sarfo, University of Calgary, Calgary, AB, Canada

    33.16 Hsp90 Regulates Binding of PPD Proteins to Dicer

    Nasser Tahbaz, University of Alberta, Edmonton, AB, Canada

    33.17 The Search for the Protein Interacting with Aggrecanase-1

    Koji Yoshida, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan

    33.18 Comparative Protein Polymorphism Analysis of Two Pike-Perches

    I. Asiful, Institute of Biophysics and Biochemistry, Russian Academy of Science, Kazan, Russia

    33.19 AMPA Receptors Are Modulated by Acetylcholinesterase

    Silvia Olivera Bravo, Instituto Clemente Estable, Montevideo, Uruguay and University of Bristol, Bristol, UK

    33.20 Identifying the Escherichia coli FtsY Binding Partners Using the Tandem Affinity Purification Protocol

    Felicia Vulcu, McMaster University, Hamilton, ON, Canada

    33.21 Numb-interacting Protein (NIP) Co-localizes with Numb and Functions in Cell-fate Determination in Drosophila Nervous System

    Hanjuan Qin, University of Western Ontario, London, ON, Canada

    33.22 Characterization of 82-kDa Choline Acetyltransferase

    Sandeep K. Gill, University of Western Ontario, London, ON, Canada

    33.23 Identification of Protein Complexes Interacting with Synaptic Protein Gap-43 by 1-, 2-D Gel/MS Analyses

    Balu Chakravarthy, National Research Council of Canada, Ottawa, ON, Canada

    33.24 Molecular Chaperones for Fibrous Proteins: Hsp47 and FKBP-65

    Vettai Ananthanarayanan, McMaster University, Hamilton, ON, Canada

    33.25 Protein Substrate Profiling of Oxidoreductase-specific Chaperones in Escherichia coli

    Jenika Binotto, University of Calgary, Calgary, AB, Canada

    33.26 Chemical In Vivo Crosslinking as a Means for Identifying Protein-Protein Interactions

    Julian Vasilescu, University of British Columbia, Vancouver, BC, Canada

    33.27 Mechanism of PrpF3 Mutations Leading to Retinitis Pigmentosa

    Juan Maria Gonzalez-Santos, University of Toronto, Toronto, ON, Canada

    33.28 BRET as a Functional Genomic Tool for Studying Protein-Protein Interactions in Living Mammalian Cells

    Fadi Hamdan, University of Montréal, Montréal, QC, Canada

    33.29 Interaction of MCM2 with RNA Polymerase II Holoenzyme

    Linda Holland, University of Guelph, Guelph, ON, Canada

    33.30 Analysis of the Conformational Transition Occurring Upon Amyloid Aggregation of the HET-S Prion Protein by Hydrogen/Deuterium Exchange Monitored by Mass Spectrometry

    Jean-Marie Schmitter, Institut Européen de Chimie et Biologie, Pessac, France

    33.31 The Leucine Zipper-like HEPTAD Repeat Domain of Transitin Interacts with the Intermediate Filament Alpha Helical Rod Domain

    Dominique Guérette, Université Laval, Sainte-Foy, QC, Canada

    33.32 A Novel System to Clone Binding Proteins of Transcriptional Activators

    Cynthia Ho, Ontario Cancer Institute, Toronto, ON, Canada

    33.33 Toward the Design of Multivalent Polypeptide Libraries for Functional Proteomics

    Andy Ng, McGill University, Montréal, QC, Canada

    33.34 Protein-Protein Interactions in the Yeast Signaling Pathways: Structures and Interactions of the Ste50-binding Domain of the MAPKKK Ste11

    Surajit Bhattacharjya, Biotechnology Research Institute, Montréal, QC, Canada

    33.35 Energetics and Specificity of Interactions within Ub[yen]Vev[yen]Ubc13 Human Ubiquitin Conjugation Complexes

    Leo Spyracopoulos, University of Alberta, Edmonton, AB, Canada

    33.36 Protein-Protein Interaction Mapping Using a Metal Chelate Reagent

    Barbara Olson, Pierce Biotechnology, Rockford, IL, United States

    33.37 Cloning of Complete cDNA for Two L1CAM Homologues in Zebrafish

    Wanyi Xiang, University of Toronto, Toronto, ON, Canada

    33.38 Structural Changes in an αβ T-Cell Receptor Upon Ligand Binding

    Craig Clements, Monash University, Clayton, Victoria, Australia

    33.39 Interaction of a Low Mobility Group Protein, LMG160, with Deoxyribonucleic Acid

    Soudabeh Fallah, University of Tehran, Tehran, Iran

    33.40 Translation Elongation Factor eEF1A, a Protein with a Potential Multiple Protein-Protein Interaction Role

    Francisco Mansilla Castaño, Aarhus University, Aarhus, Denmark

    33.41 Proexosite I Ligands as Probes for the Study of Prothrombin Activation

    Robson Queiroz Monteiro, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

  • 34. Poster Session 9

    Proteomes of Plants

    34.1 Extraction, Composition, Solubility and Electrophoresis Patterns of Storage Proteins in Aleuron Grains (Protein Bodies) and Extraction of RNA Isolated from Pistachio Nuts (Pistachio vera L.) Ohadi Variety of Kerman-Iran

    Akram Sadat Tabatabaee-Panah, Islamic Azad University, Tehran, Iran

    34.2 Two Dimensional Gel Electrophoresis and Analysis of Polypeptides in Developmental Stages of Olive Fruit Ripening

    Nasrin Motamed, Tehran University, Tehran, Iran

    34.3 Evaluation of the Effect of Salinity Stress on Saponin Contents in Bellis perrenis L.

    Elham Attaran, Teacher Training Univesity, Tehran, Iran

    34.4 Possible Physiological Role of Lectins in the Process of Germination of Bean Seeds (Phaseolus vulgaris L.).

    Nataliya Kovalchuk, Institute of Botany, Kyiv, Ukraine

  • 35. Poster Session 10

    Rational Drug Design

    35.1 Structure Determination of Methylthioribose Kinase: Target for Rational Drug Design in Methionine Salvage Pathway

    Shao-Yang Ku, Hospital for Sick Children and University of Toronto, Toronto, ON, Canada

    35.2 Peptide Effect on the Half-life of the Chimeric Erythropoietin

    Dong-Eok Lee, Ichon, South Korea and KAIST, Taegon, South Korea

    35.3 Testis LDH as Target for Immunoliposomes

    Ranjna Dutta, Northwestern University, Evanston, IL, United States

    35.4 The Design of Self-assembling, Peptide-based Delivery Vehicles Based on the Human p53 Tetramerization Domain

    Michael Sung, University of Toronto, Toronto, ON, Canada

    35.5 Quantitating the Dissociation Kinetics of Transient Peptide-Protein Complexes by Use of Peptide 15N NMR Relaxation Dispersion Spectroscopy

    Dmitri Tolkatchev, Biotechnology Research Institute, Montréal, QC, Canada

    35.6 Novel Natural Triterpene Derivatives as Specific Inhibitors of HIV-1 Integrase

    Elena Semenova, State Research Ctr of Virology & Biotechnology “Vector”, Koltsovo, Russia

  • 36. Poster Session 11

    The Machinery for Protein Degradation

    36.1 Novel Ring Finger Proteins Interacting with the Ubc13-UEV Heterodimer

    Timothy Thomson, Institut de Biologia Molecular de Barcelona, Barcelona, Spain

    36.2 Isolation, Molecular Characterization and Regulation of Cysteine Protease Gene in Gladiolus grandiflora

    Ajay Arora, University of Tsukuba, Tsukuba, Ibaraki, Japan

    36.3 PCNA Turnover in Cell Cycle and Involvement in DNA Repair Is Linked to Modification by Ubiquitin

    Stanislav Naryzhny, Neorcc, Sudbury, ON, Canada

    36.4 Characteristics of Trypsin-like Proteinase from the Midgut of the Yellow Mealworm

    Elana Elpidina, Moscow State University, Moscow, Russia

    36.5 Isolation of Single Chain Antibodies by Phage Display Against Age-dependent Glycation on the a-7 Subunit of the Proteasome

    Regina Gonzalez-Dosal, Aarhus University, Aarhus, Denmark

    36.6 PHEX, an Enzyme of M13 Family Has a Distinct Specificity and Cleaves Peptides Derived from FGF-23 and MEPE

    Marcelo Campos, Escola Paulista De Medicina, Unifesp, SÃo Paulo, Brazil

    36.7 Study of S3-S3′ Subsite Specificity of Recombinant Human Cathepsin K and Development of Selective Internally Quenched Fluorescent Substrates

    Marcio Fernando Madu Alves, Universidade Federal de SÃo Paulo, SÃo Paulo, Brazil

    36.8 Deglycosylation and Ubiquitination Precede Complete Retro-translocation of a Lumenal ERAD Substrate

    Claudia Kitzmüller, University of Vienna, Vienna, Austria

  • 37. Poster Session 12

    Education in the Molecular Life Sciences

    37.1 Structural Changes of DNA Induced by Caffeine

    Mahvash Jafari, University of Tehran, Tehran, Iran

    37.2 Using Molecular Markers to the Study of Endangered Natural Populations of the White Shrimp (L. schmitti) in Cuba

    Yaisel Juan Borrell Pichs, University of Havana, Ciudad de la Habana, Cuba

    37.3 Pedagogical Methodology for the Evaluation of Online Biochemistry Courses

    Jorge Joel Reyes-Méndez, Universidad Autónoma Metropolitana, DF, Mexico

    37.4 Graduate Teaching Internships: A Means of Enhancing Science Instruction and Research at Predominantly Undergraduate Institutions in North Dakota

    Katherine Sukalski, University of North Dakota, Grand Forks, ND, United States

    37.5 Improving Accessibility and Participation of Students of Small Baccalaureate and Tribal Colleges to Research Seminars Using an Interactive Video Network (IVN)

    Hilde Van Gijssel, Valley City State University, Valley City, ND, United States

    37.6 Molecular Study of Fusarium Oxisporum Isolated by RAPD

    Behrang Alani, Drug Applied Research Center, Tabriz, Iran

  • 38. Poster Session 13

    Transport, Trafficking and Endocytosis

    38.1 Studies on DNA-Liposome Interactions

    Pouneh S. Pourhosseini, Institute of Biochemistry & Biophysics, Tehran University, Tehran, Iran

    38.2 Accumulation of hsc73 in Nuclei Upon Heat Stress Depends on a Non-classical Nuclear Signal

    Huanhuan Gao, McGill University, Montréal, QC, Canada

    38.3 Crystal Structures of Importin Alpha Bound with Bipartite and Phosphorylated NLSs

    Marcos Fontes, Universidade Estadual Paulista, Botucatu, Brazil

    38.4 Structural Studies of the Nuclear Membrane During Cereal Seeds Germination

    Lia Minasbekyan, Yerevan State University, Yerevan, Armenia

    38.5 Phosphatidyl Inositol (4,5) Biphosphate Is Required for Fusion of COPI Derived Vesicles

    Frédéric Laporte, McGill University, Montréal, QC, Canada

    38.6 Barley Alpha-amylase Expressed in AtT20 Cells Is N-glycosylated and Its Secretion Retarded by a Lectin-like Activity in a Post TGN Compartment

    Helena Senta, Université de Sherbrooke, Sherbrooke, QC, Canada

    38.7 Nuclear Transport of Heat Shock Proteins in Stressed Cells

    Mohamed Farouk Kodiha, McGill University, Montréal, QC, Canada

    38.8 RNA-interference Reveals Distinct Roles for Plasma Membrane Syntaxins in Epithelial Fluid Secretion

    Ross Mclennan, University of Glasgow, Glasgow, United Kingdom

    38.9 PKC-delta Dependent Cleavage and Nuclear Translocation of Annexin-I by Phorbol 12-Myristate 13-Acetate Äï

    Doe Sun Na, University of Ulsan College of Medicine, Seoul, South Korea

    38.10 Investigations into the Molecular Basis of Protein Secretion from the Salivary Glands of the Tick, Ornithodoros savignyi (Acari: Argasidae)

    Christine Maritz, University of Pretoria, Pretoria, South Africa

    38.11 Kdg2, a Novel Multidomain Protein Involved in the Regulation of Recycling Endosomal Trafficking

    Hidekazu Fukuda, Tokyo Institute of Technology, Yokohama, Japan

    38.12 Interaction of Carbonic Anhydrase II and the C-terminus of the Human SLC26A6 Bicarbonate Transporter

    Bernardo Alvarez, University of Alberta, Edmonton, AB, Canada

    38.13 Osmo-responsive Amino Acid Transporter in Pacific Oyster

    Haruhiko Toyohara, Kyoto University, Kyoto, Japan

    38.14 ELFMF’s Increase the Alveolar Epithelial Tight Junction Permeability by Effect on Protein Kinase C Signal Transduction

    Afshin Ebrahimpour, Shahid Chamran University, Ahwaz-Mollasani, Iran

    38.15 Membrane Transport Without Receptors? Interaction of Different Cyclosporines and Silymarines with the Lipidic Part of Hepatocyte Plasma Membrane

    Jiri Sebestian, University of South Bohemia, Ceske Budejovice, Czech Republic

    38.16 The Phospholipid Binding Domain of Oxysterol Binding Protein (OSBP) Related Protein 1 (ORP1) Is Not Sufficient to Regulate the Sec14p Pathway

    Gregory Fairn, Dalhousie University, Atlantic Research Centre, Halifax, NS, Canada

    38.17 Structure of the C-terminal Domain of SecA

    Brian Dempsey, University of Western Ontario, London, ON, Canada

    38.18 K-Ras Regulation of Anchorage-independence and Antigen Expression in Human Prostate Cell Culture

    Myron Williams, Clark Atlanta University, Atlanta, GA, United States

    38.19 Intracellular Trafficking of Scavenger Receptor BI

    Chris Harder, University of Ottawa Heart Institute, Ottawa, ON, Canada

    38.20 Nuclear Localization of the Mineralocorticoid Receptor Is Determined by Multiple Dispersed Signals

    Rhian Walther, The Ottawa Health Research Institute and the University of Ottawa, Ottawa, ON, Canada

    38.21 Membrane Vesicles from Helicobacter pylori as a Potential In Vitro Source of the Vacuolating Cytotoxing

    Guadalupe Ayala, Instituto Nacional de Salud Publica, Cuernavaca, Mexico

    38.22 Hepatocytes Hexose Uptake at the Hyperglycemia State

    Umerov Oybek, Scientific Institute of Endocrinology, Tashkent, Uzbekistan

    38.23 Insulin Receptor Trafficking and Signaling In Vivo: Effects of V-ATPase Inhibition

    Nicolas Bilodeau, Université Laval, Québec, QC, Canada

    38.24 Control of Insulin Receptor Trafficking and its Response by the Protein Tyrosine Phosphatase SHP-1

    Annie Fiset, Université Laval, Québec, QC, Canada

    38.25 Two Kinesin-like Motor Proteins, KIF16B and KIF16B’, and their Possible Role in the Movement of Early and Late Endosomes

    Alicia Cabezas, Norwegian Radium Hospital, Oslo, Norway

    38.26 Caveolar Endocytosis Is Involved in the Intracellular Delivery of HIV-1-TAT Fusion Proteins

    Aldo Ferrari, NEST-INFM and Scuola Normale Superiore, Pisa, Italy

    38.27 Nuclear Localization Signals of Kaiso and the Catenin p120ctn

    Kevin Kelly, McMaster University, Hamilton, ON, Canada

  • 39. Poster Session 14

    Antibody Engineering

    39.1 Microfluidic Technology Applied to Quality Control of Antibodies

    Tanja Neumann, Agilent Technologies Deutschland GmbH, Waldbronn, Germany

    39.2 Isolation of Neisseria gonorrhoeae-specifc Antibodies from a Phage Display Library

    Sanjiv Rughooputh, University of Westminster, London, United Kingdom

    39.3 Selection of Anti-ssDNA Chicken scFVs from a Non-immunized Animal Phage Display Combinatorial Library

    Andrea Maranhao, Universidade de Brasília, Brasília, Brazil

    39.4 Phage Display Antibody Against Gill Proteins of the Tropical Clam

    Jean-Philippe Gourdine, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe, France

    39.5 A General Method to Design Primers for the Amplification

    Essono Sosthène Severin, Service de Pharmacologie et d’Immunologie, DRM/DSV, CEA-Saclay, Gif-sur-Yvette, France

    39.6 From Genes to Intracellular Antibodies: Dissecting the Proteome with Splint, a Single Pot Library of Stable Antibodies

    Michela Visintin, Lay Line Genomics, Rome, Italy

    39.7 Humanization and Binding Activity of Humanized/Murine Hybrid Anti-CD3 scFVs

    Marcelo Brigido, Universidade de Brasilia, Brasilia, Brazil

    39.8 Comparing Methods to Obtain Antibody Probes for Proteomic Analysis

    Daniel Laune, CNRS UMR 5160, Montpellier, France

  • 40. Poster Session 15

    Enzyme Catalysis and Regulation

    40.1 Multiple Conformations of Adenylate Kinase in Native State

    Xiang Rong Sheng, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R. China

    40.2 RNase Irp3, a Unique Precursor of Extracellular RNases of Irpex lacteus

    Kazuko Ohgi, Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Shinagawa-ku, Tokyo, Japan

    40.3 Toxic Effect of Cadmium Chloride on Lipid Peroxidation, Blood Hematology, Biochemical Parameters and Semen Quality of Male Rats: Vitamin E and β-Carotene as Antioxidants

    Fatma El-Demerdash, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt

    40.4 Adsorptive Immobilization of Glutamate Dehydrogenase in an Allosterically-activated Conformation

    Zahra Salemi, Institute of Biochemistry & Biophysics, Tehran University, Tehran, Iran

    40.5 The Effect of N-acetylimidazole on the Structure, Stability and Suicide Substrate Inactivation of Mushroom Tyrosinase

    Farhad Karbassi, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    40.6 The Effect of Some Amino Acids on the Structure and Activity of Carbonic Anhydrase

    Naghme S. Sarraf, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    40.7 The OMP Dimer Is a Metabolically Active Form

    Jae Hyung Koo, University of Maryland School of Medicine, Baltimore, MD, United States

    40.8 Kinetic Mechanism of Pyruvate Phosphate Dikinase of Entamoeba histolytica

    Marcela Varela, Universidad Nacional Autónoma de México, Cd. de México

    40.9 Homology Modelling and Molecular Dynamics Simulation of Beta-Galactosidase from Antarctic Bacterium Arthrobacter sp. C2–2

    Vojtech Spiwok, Ict Prague, Prahue, Czech Republic

    40.10 Variation in Caffeine Content of Tealeaves Due Cellulase Activity

    Reyhaneh Sariri, Gilan University, Rasht, Iran

    40.11 Properties of Recombinant Human Pyruvate Dehydrogenase Kinase

    Mary Maj, The Hospital for Sick Children, Toronto, ON, Canada

    40.12 Enzymatic Analysis of Mutant PabB Protein in E. coli

    Michele Joike, University of Illinois–Chicago, Chicago, IL, United States

    40.13

    An Ionic Peroxidase Contributes to Enzymic Browning in Dioscorea esculenta Tubers

    Joy Okpuzor, Department of Cell Biology and Genetics, University of Lagos, Lagos, Nigeria

    40.14 Pre-steady-state Kinetic Analysis of Interaction of Repair Enzymes Fpg Protein and hOgg1 with DNA Substrates

    Olga Fedorova, Institute of Bioorganic Chemistry, Novosibirsk, Russia

    40.15 Inhibition of Pyroglutamyl Aminopeptidase II Activity by HcPI, a Natural Inhibitor from the Marine Annelide Hermodice carunculata

    Isel Pascual, Faculty of Biology, University of Havana, Ciudad de la Habana, Cuba

    40.16 Combined Doses of Vanadate and Fenugreek Correct the Elevated Levels of Gluconeogenic Enzymes in Alloxan-diabetic Rat Liver

    Sameer Mohamad, Jawaharlal Nehru University, New Delhi, India

    40.17 The Role of Endogenous Aflatoxin, Glutathione S-Transferase and Reduced Glutathione in Bioregulation of Aflatoxin Synthesis in Aspergillus parasiticus

    Mehdi Razzaghi Abyaneh, Pasteur Institute of Iran, Tehran, Iran

    40.18 Quercetin as a Novel Highly Specific Aromatase Inhibitor

    Inna Yasinska, Mechnikov Odessa National University, Odessa, Ukraine

    40.19 Molecular Cloning and Expression of Oryza sativa Glutathione S-Transferase in Escherichia coli

    Kwang-Hoon Kong, Chung-Ang University, Seoul, South Korea

    40.20 Theoretical and Experimental Studies on the Effect of the Micro-environment on the pKa of Lys213 in Saccharomyces cerevisiae Phosphoenolpyruvate Carboxykinase

    Alejandro Yevenes, Universidad de Santiago de Chile, Santiago, Chile

    40.21 Cloning of a Fungal Chitin Eacetylase Gene in E. coli and Pichia pastoris

    Binesh Shrestha, Asian Institute of Technology, Pathumthani, Thailand

    40.22 Presence of Eukaryotic-like Ser/Thr Protein Kinases and Protein Phosphatases in Salmonella typhi

    Sio Mei Lai, McGill University, Montréal, QC, Canada

    40.23 Phosphorylation Pattern of RNA Polymerase II Carboxyl-terminal Domain by Three Cyclin-dependent Kinases

    Reena Pinhero, University of Guelph, Guelph, ON, Canada

    40.24 Investigating the Mobile Regions in E. coli Citrate Synthase by NMR Spectroscopy

    Kajal Choudhary, University of Manitoba, Winnipeg, MB, Canada

    40.25 Solution Structure of 2′,3′-Cyclic Nucleotide 3′-Phosphodiesterase

    Kalle Gehring, McGill University, Montréal, QC, Canada

    40.26 A Novel Uracil-DNA Glycosylase Family and its Action Mechanism

    Eun Kyoung Im, Yonsei University College of Medicine, Seoul, South Korea

    40.27 Toxicity by Peroxisome Proliferators and its Protection in Rat Hepatocyte Cultures

    Hiroko Kawano, Kobe Gakuin University, Kobe, Japan

    40.28 Antioxidant Effect of Vitamin E and Selenium on Lipid Peroxidation, Enzyme Activities and Biochemical Parameters in Rats Exposed to Aluminium

    Fatma El-Demerdash, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt

    40.29 Determinants in the Pro-domain of Adamts-1 and Adamts-9 Are Involved in Their Maturation and Secretion

    Jean-Michel Longpré, Université de Sherbrooke, Sherbrooke, QC, Canada

    40.30 Beta-diketo Derivatives as Mimics of the Aldolase Catalysed Reaction?

    Nicolas Chabot, Université Paul Sabatier, Toulouse, France

    40.31 Structure of the Enamine Intermediates Trapped in Rabbit Muscle Aldolase

    Miguel St-Jean, Université De Montréal, Montréal, QC, Canada

    40.32 Amino Acid Replacements in the Allosteric Binding Site of Type II Citrate Synthase Lead to Changes in Quaternary Structure: Assessment by Electrospray Time-of-Flight Mass Spectrometry

    Harry Duckworth, University of Manitoba, Winnipeg, MB, Canada

    40.33 Effect of Chemical Modification of Lysine Residues on Activity and Stability of Añ-Amylase

    M. Javed Asad, Independant Medical College Jinah Colony, Faisalabad, Punjab, Pakistan

    40.34 Biosynthesis of Extracellular Lipase by Aspergillus niger

    Beda Dahal, Himalayan College of Agricultural Sciences and Technology (HICAST), Gatthabhar, Nepal

    40.35 Cleavage of Different RNA Targets by a Small, Structurally Versatile Deoxyribozyme

    Rani Cruz, McMaster University, Hamilton, ON, Canada

    40.36 Action of Mre11 and Rad50 at Radiation-induced Strand Break Termini

    Aghdass Rasouli-nia, Cross Cancer Institue, Edmonton, AB, Canada

    40.37 Activation of Phosphoenolpyruvate Carboxykinase from Escerichia coli by Ca2+ and Mechanism of Desensitisation by Trypsin

    Hughes Goldie, University of Saskatchewan, Saskatoon, SK, Canada

    40.38 Studying the Interactions of PC1/3 with its Propeptide Through Site Directed Mutagenesis

    Nadia Rabah, Institut De Recherches Cliniques De Montréal, Montréal, QC, Canada

    40.39 Protein Kinase CK2 Catalyses Tyrosine Phosphorylation in Mammalian Cells

    Greg Vilk, University Of Western Ontario, London, ON, Canada

    40.40 The Kinetic Properties of the Heterogeneous Enzymatic Assay as a Model System of Enzyme Quasi-saturating Behavior

    Omar Gutierrez-Arenas, University of Havana, Faculty of Biology, Havana City, Cuba

    40.41 Mechanism and Biological Significance of Reactions and Events Mediated by Myeloperoxidase in the Xenobiotic Metabolism and Disposition Pathways of Phagocytes, Neutrophils

    Takeshi Odajima, Health Sciences University of Hokkaido, Hokkaido, Japan

    40.42 Degradation of the Proteinic Microbial Metabolite Toxins, Staphylococcal Alpha Toxin and Enterotoxin B, by the Myeloperoxidase System

    Mihoko Onishi, Health Sciences University of Hokkaido, Hokkaido, Japan

    40.43 Characterization and Specifity of a Barley (H. vulgare) Metalloproteinase

    Nasser Ghaemi, University of Tehran, Tehran, Iran

    40.44 Characterization and Biochemical Studies of an Alkaline Protease, Use in a Biodetergent Assay

    Mohamed Nejib Marzouki, National Institute of Applied Sciences and Technology Insat, Tunis, Tunisia

    40.45 Evaluation of Alpha Glutathione-S-Transferase as Biochemical Marker of Hepatocellular Damage in Chronic Hepatitis Virus Infection

    Esmat Ashour, National Research Centre, Cairo, Egypt

    40.46 Withdrawn

    40.47 Investigation of the Biochemical Changes Associated with Ex Vivo and In Vitro Developmental Processes in Dioscorea spp.

    Kathleen Lobban, University of the West Indies, Kingston, Jamaica

    40.48 Domain-Domain Interactions in the Bifunctional Aminoglycoside Antibiotic Resistance Enzyme AAC(6′)-APH(2′′)

    David Boehr, McMaster University, Hamilton, ON, Canada

    40.49 Spontaneous Deamidation of the Asn Residue in the Inactive D165N Mutant of Clostridium Symbiosum GDH Leads to Reactivation of the Enzyme

    Francesca Paradisi, University College Dublin, Dublin, Ireland

    40.50 Horse Butyrylcholinesterase Inhibition with Ethopropazine Enantiomers: Temperature Influence on Stereoselectivity

    Goran Sinko, Institute for Medical Research & Occupational Health, Zagreb, Croatia

    40.51 Properties of the Main Laccase Produced by the White-Rot Fungus Pleurotus pulmonarius on Solid State Medium

    Adriana Zilly, University of Maringá, Maringá, Paraná, Brazil

    40.52 The Potentiate Effect of Nitric Oxide Donors After Botulinum Toxin Application

    Mariusz Madalinski, St. Wojciech Adalbertus Hospital, Gdansk, Poland

    40.53 Composition and Properties of Partial Hydrolysis Mixtures of Phosphoryl Chloride as Determined by 18-O Mass Spectrometry and 31-P NMR: Implications for Their Use as Phosphorylating Agents for the Synthesis of Phosphate Esters

    Robert Mitchell, Wayne State University, Detroit, MI, United States

  • 41. Poster Session 16

    Apoptosis

    41.1 Induction of Apoptosis in Alveolar Macrophages Exposed to Lead Nitrate and Modulation Effect of Indomethacin and Caffeine

    Ashraf Shabani, University of Azzahra, Tehran, Iran; Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    41.2 Ubiquitin

    Morihiko Nakamura, Shimane Medical University, Izumo, Belarus

    41.3 Expression of Telomerase, Bcl-2, Bcl-XL and Survivin in Relation to Loss of Apoptosis in HNSCC: Correlation with Clinico-pathological Features

    Himani Sharma, All India Institute of Medical Sciences, New Delhi, India

    41.4 Insecticide Induced Expression of 70 kDa Heat Shock Protein in Transgenic Drosophila Melanogaster: Correlation Between Hsp70 Expression and Apoptosis

    Indranil Mukhopadhyay, Industrial Toxicology Research Centre, Lucknow, Uttar Pradesh, India

    41.5 Induction of Apoptosis by Chlorin e6 and Porphyrins

    Sona Ghambaryan, Institute of Biotechnology, Yerevan, Armenia

    41.6 Combination of DNA Laddering and Annexin-V and Caspase Assays on One System—Multiple Apoptosis Parameters Analysis with a Microfluidic Chip-based System

    Tanja Neumann, Agilent Technologies Deutschland GmbH, Waldbronn, Germany

    41.7 Myc-dependent Changes in Bax Membrane Topology

    Matthew Annis, McMaster University, Hamilton, ON, Canada

    41.8 Determining the Role of Apoptosis in Retinoblastoma Development

    Helen Dimaras, Ontario Cancer Institute/Princess Margaret Hospital, University Health Network, Toronto, ON, Canada and University of Toronto, Toronto, ON, Canada

    41.9 rAAV-mediated Trail Gene Therapy Suppresses Liver Metastatic Tumors

    Dexian Zheng, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing, P.R. China

    41.10 Human Cartilage Glycoprotein 39 (HC-gp39) Inhibits Stress-induced Signaling Pathways and Promotes Connective Tissue Cell Survival

    Hua Ling, Shriners Hospital, McGill University, Montréal, QC, Canada

    41.11 Induction of p53-dependent Apoptosis by Polyplex of p53 cDNA and Dendrosome as a Novel Nonviral Vector with High Efficiency in Human Lymphomic and Leukemic Cells

    Mohammad Massumi, Tehran University, Tehran, Iran

    41.12 Effects of Hepatitis B Virus and IAPs on Hepatoma, HBx Promotes the Expression of Survivin

    Xiaodong Zhang, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin, P.R. China

    41.13 Expression of Bcl-2 Family and Hepatitis B Virus X Protein in Hepatoma Tissues

    Nan Dong, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin, P.R. China

    41.14 Analysis of Posttranslational N-myristoylation of Caspase-cleavage Product of Cytoskeletal Actin

    Toshihiko Utsumi, Yamaguchi University, Yamaguchi, Japan

    41.15 Involvement of Reactive Oxygen Species in Ca2+-induced Membrane Permeability Transition of Mitochondria

    Kozo Utsumi, Institute of Medical Science, Kurashiki Medical Center, Kurashiki, Japan

    41.16 Mitochondrial Permeability Transition in the Signaling Pathway of Apoptosis: Inhibition by Hydroxytamoxifen

    Carla Cardoso, University of Coimbra, Coimbra, Portugal

    41.17 Higher Tolerance to Oxidative Stress in Fibroblasts from Alzheimer’s Patient

    Jafar Naderi, University of Windsor, Windsor, ON, Canada

    41.18 Bim Induced Confromational Change in Bcl-2 Membrane Topology

    Paulina Dlugosz, McMaster University, Hamilton, ON, Canada

    41.19 Role of Mitochondria in Neuronal Cell Death by Oxidative Stress; Neuroprotection by Coenzyme Q10

    Mallika Somayajulu, University of Windsor, Windsor, ON, Canada

    41.20 Effects of 4,977 bp-deleted Mitochondrial DNA on UV-induced Apoptosis of Human Cells

    Chun Yi Liu, National Yang Ming University, Taipei, Taiwan

    41.21 Bcl-2 Family and oxLDL Mediated Macrophage Survival

    Shih Wei Wang, University of British Columbia, Vancouver, BC, Canada

    41.22 Study of Hormonal Regulations of Apoptosis

    Zulaykho Shamansurova, Scientific Institute of Endocrinology, Tashkent, Uzbekistan

    41.23 Casodex Treatment Induces Hypoxia-related Gene Expression in the LNCaP Prostate Cancer Progression Model

    Velliyur Gopalakrishnan, University of Nebraska Medical Center, Omaha, NE, United States

    41.24 The Role of ICAD-isoforms in the Formation and Intracellular Distribution of CAD

    Sebastian R. Scholz, Justus-Liebig-University Giessen, Giessen, Germany

    41.25 Linking Lovastatin-induced Apoptosis in Multiple Myeloma Cells to Genetic Factors

    Wendy W. L. Wong, University of Toronto, Toronto, ON, Canada; Ontario Cancer Institute, University Health Network, Toronto, ON, Canada

    41.26 Diabetes Induces Apoptosis in Lymphocytes

    Rui Curi, Institute of Biomedical Sciences/University of SÃo Paulo, SÃo Paulo, Brazil

  • 42. Poster Session 17

    Protein Folding and Misfolding

    42.1 On the Kinetic and Thermodynamic Perspectives of Protein Folding and Misfolding Mechanisms–Biomedical Implications

    Nigil Satish Jeyashekar, University of Mississippi, University, MS, United States

    42.2 Folding Pathway Mediated by an Intramolecular Chaperone: The Subtilisin Propeptide Is Optimized To Be Intrinsically Unstructured

    Ezhilkani Subbian, Oregon Health and Sciences University, Portland, OR, United States

    42.3 Preparation of High Amounts of Recombinant E1B Protein of Adenovirus 12 in Baculovirus Expression System and its Application in Alternative Splicing

    Ali Reza Roostaee, Tarbiyat Modarres University, Tehran, Iran

    42.4 Preparation and Characterization of an Intermediate Form of Bacillus subtilis Alpha-Amylase

    Hamid Reza Karbalaei-Heidari, University of Tehran, Tehran, Iran

    42.5 Interaction of Glutamate Dehydrogenase with Alkyl-substituted Sepharose 4B

    Marjan Sabbaghian, Institute of Biochemistry and Biophysics, Tehran, Iran

    42.6 Adsorptive Immobilization of Amyloglucosidase on Concanavalin A-Sepharose 4B: Enhancement of Activity and Stability

    Javad Jafary Aghdam, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    42.7 Circularizing Proteins

    Pavel Prosselkov, Research School of Chemistry, Australian National University, Canberra, ACT, Australia

    42.8 Heteronuclear NMR Studies of 13C/15N Labeled HIV-1 Tat

    Shaheen Shojania, University of Manitoba, Winnipeg, MB, Canada

    42.9 Automated Refolding of Protein from Inclusion Bodies

    Lori Kohlstaedt, Proteomtech, Inc., Emeryville, CA, United States

    42.10 Reactivation Kinetics of Homodimeric Triosephosphate Isomerase

    Viviana Zomosa-Signoret, Instituto de Fisiología Celular, UNAM, Mexico City, Mexico

    42.11 Structural Stabilization of baL, bab and (ba)2 Substructures of Triose Phosphate Isomerase (TIM) from a Hyperthermophilic Archaeon Pyrococcus furiosus

    Sourav Mukherjee, Institute of Microbial Technology (imtech), Chandigarh, India

    42.12 Nucleotide Affinity Cleavage as a Potential Tool of Structural Proteomics: Inhibitors of the Hsp90 Chaperone as an Example

    Peter Csermely, Semmelweis University, Budapest, Hungary

    42.13 The Structures of Periplasmic Proteins in Escherichia coli Are Highly Sustainable to Denaturing Conditions

    Chang Zengyi, Tsinghua University, Beijing, P.R. China

    42.14 The Role of Hsp90/Hsp90 Complex in the Degradation of CFTR in S. cerevisiae

    Robert Youker, University of Pittsburgh, Pittsburgh, PA, United States

    42.15 A Rapid Method for Continuously Monitoring the Folding State of Actin Using Absorbance Spectrophotometry

    Braden Sweeting, University of Guelph, Guelph, ON, Canada

    42.16 The Structure and Mobility of the Hydrogen Bonds Net of the Surface Water in the Ligand-Receptor Interaction Process

    Svetlana Rogacheva, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia

    42.17 The Activity of Chaperon’s Low-molecular Analogs

    Era Popyhova, Saratov State University, Saratov, Russia

    42.18 Induction of Molten Globule-like Structures Upon Modification of Glucose Oxidase

    Saman Hosseinkhani, College of Basic Sciences, Tarbiat Modarres University, Tehran, Iran

    42.19 Amyloid-like Fibril Formation and Cytotoxicity of a Myoglobin Mutant

    Clorinda Malmo, Seconda Università degli Studi di Napoli, Napoli, Italy

    42.20 Structural and Folding Basis of Intracellular Protein Targeting of AGT

    Xiaoxuan Zhang, University College London, London, United Kingdom

    42.21 Structural Comparison of an Unstable Wild Type SH3 Domain and its Stable Mutant

    Irina Bezsonova, University of Toronto, Toronto, ON, Canada; Hospital for Sick Children, Toronto, ON, Canada

    42.22 Identification and Characterization of a Novel Molecular-Recognition and Self-Assembly Domain Within the Islet Amyloid Polypeptide

    Sharon Gilead, Tel-Aviv University, Tel-Aviv, Israel

    42.23 HOP Functions as a Mediator for Conformational Changes in the hsp70-Hop-hsp90 Molecular Chaperone Complex

    M. Patricia Hernández, University of Toronto, Toronto, ON, Canada

    42.24 A Prion Protein Folding Intermediate Stabilized by Hydrostatic Pressure and Low Temperature

    Samantha Martins, Universidade Federal de Rio de Janeiro, Rio de Janeiro, Brazil

    42.25 The Ability to Restoration of Native Structure in High Concentrated Globular Protein-Water Systems After the Thermal Treatment: DSC Study

    N. A. Grunina, Research Institute of Physics, St. Petersburg State University, St. Petersburg, Russia

  • 43. Poster Session 18

    Functional Proteomics

    43.1 Does cis-Element Absorb trans-Element by EMFs?

    Hamid Hadi Alijanvand, Shahid Chamran University, Isfahan, Iran

    43.2 Comparative Proteomics of Integral and Peripheral Membrane Proteins from Human and Rat Red Blood Cells (RBC)

    Sébastien Taurin, Research Centre, University of Montreal Hospital (CHUM-Hotel-Dieu), Montréal, QC, Canada

    43.3 DNA Aggregation by an Archaeal DNA Binding Protein: Sac10b and Its Novel DNA Nicking Activity

    Tangirala Suryanarayana, University of Hyderabad, Hyderabad, India

    43.4 Magnetic Bead Based High Throughput Isolation of Polyhistidine-tagged Proteins for Purification and Target Screening

    Stine Bergholtz, Dynal Biotech Asa, Oslo, Norway

    43.5 Surface Plasmon Resonsance as a Tool to Identify Binding Partners in Conjunction with Mass Spectrometry

    Jimmy Page, Biacore, Inc., Piscataway, NY, United States

    43.6 Integrated Approaches in Functional Proteomics of Yeast: A Comparison Between Two Protein Extraction Methods Used for Study of Protein Complexes

    S. H. (Amir) Hashemi, Goteborg University, Lundberg Laboratory, Gothenburg, Sweden

    43.7 A Beetle Odorant-binding Protein Family: Sampling Isoform Diversity by a Combined Mass-Spectroscopy and cDNA Cloning Approach

    Laurie Graham, Queen’s University, Kingston, ON, Canada

    43.8 A Novel Method for Preparing and Analyzing Membrane Proreome

    Kenji Tanaka, Mitsubishi Pharma Corporation, Hirakata, Osaka, Japan

    43.9 Differential Display Proteomic Analysis of HEK293 Cells Transfected with Amyloid Precursor Protein Gene

    Ji Jianguo, Proteomic Research Group, Beijing, P.R. China

    43.10 Preparation and Characterization of Proteoliposome for Functional Proteomics of Membrane Proteins

    Koji Munechika, Mitsubishi Pharma Corporation, Hirakata, Osaka, Japan

    43.11 Identification of Protein Kinase C Isoform-specific Phosphorylation Sites on Human Choline Acetyltransferase by Mass Spectrometry

    Tomas Dobransky, Robarts Research Institute, London, ON, Canada

    43.12 Investigation of the Yeast Hsp90 Complex Using Proteomics Approaches

    Rongmin Zhao, University of Toronto, Toronto, ON, Canada

    43.13 Human Coproporphyrinogen Oxidase (CPO): Biochemical Characterization of Wild-type Enzyme and its Naturally Occurring Mutant Forms

    Ivan Mikula, First Faculty of Medicine, Charles University, Prague, Czech Republic

    43.14 Host Cell Response to Listeria monocytogenes Invasion

    Matthias Trost, GBF, German Research Centre for Biotechnology, Braunschweig, Germany

    43.15 Harnessing the Proteome

    Joshua LaBaer, Harvard Institute of Proteomics, Harvard Medical School, Cambridge, MA, United States

    43.16 Reverse-proteomic Analysis of Rho GTPase Function and Regulation in C. elegans

    Sarah Jenna, McGill University, Montréal, QC, Canada

    43.17 In-silico Functional Proteomics for Compound Profiling and Disease Diagnosis

    Adesh Kaul, Genedata Inc., Waltham, MA, United States

    43.18 Monitoring of Regulatory Protein Redistribution Following Subcellular Proteome Extraction

    Robertus Hendriks, E.merck Darmstadt, Darmstadt, Germany

    43.19 Quantitative Protein Expression Analysis and Determination of Amino Acid Precursor Pool Enrichment in Stem Cells Performed with Stable Isotope Amino Acid In Vivo Labelling and MALDI-TOF-MS

    André Schrattenholz, Proteosys AG, Mainz, Germany

    43.20 Proteomic Survey of PKG-Targets Reveals a Novel Regulator of Smooth Muscle Contractility

    Justin Macdonald, University of Calgary, Calgary, AB, Canada

  • © 2003 The American Society for Biochemistry and Molecular Biology

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