Reviews & Perspectives
Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
In Brief Advancements in MS-based proteomics have increased the study of synaptic proteins using neuroproteomics. The development of proximity, genetic labeling and bio-orthogonal amino acid labeling approaches now allow for the study of synaptic protein–protein interactions and protein signaling dynamics. In this review, we highlight studies from the last 5 years, with a focus on synapse structure, composition, functioning, or signaling and finally discuss some recent developments that could further advance the field of neuroproteomics.Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches
Proximity-dependent biotinylation approaches such as BioID and APEX overcome classical limitations of biochemical purification and have gained widespread use in recent years for revealing cellular neighborhoods. Here we focus on the structural diversity and mechanisms of the two classes of enzymes, biotin protein ligases and peroxidases, and discuss current and emerging applications of these enzymes for proximity dependent biotinylation. We provide guidelines for enzyme selection and experimental design for performing and interpreting proximity-dependent biotinylation experiments.
