Special Issue: Glycoproteomics

Coordinating Editors: Gerald Hart and Lance Wells (guest)

Special Issue: Glycoproteomics

In 2013, we served as guest editors for the Glycomics special issue where the Athens guidelines for glycomic analyses were first revealed that have since been adopted by multiple journals. At that moment in time, we could not have easily conceived of a special issue dedicated to glycoproteomics. However, just 8 years later, we find ourselves presenting a special issue of Molecular & Cellular Proteomics on Glycoproteomics that introduces the reader to the recent explosion in front-end enrichment methods, analytical approaches, and back-end software solutions dedicated to glycoproteomics. This special issue includes nine review articles and nine research articles that introduce the reader to the exciting, challenging, and rapidly evolving field of glycoproteomics, which is so highly dependent on MS.

Glycoproteomics has been front and center this past year as scientists tackled the COVID-19 pandemic, given that the spike glycoprotein of SARS-CoV-2, which is often the basis for antibody and vaccine therapeutics, is a trimer glycoprotein consisting of 66 occupied N-linked glycosylation sites, and that the host receptor, ACE2, is also heavily glycosylated. Thus, an issue devoted to understanding this heterogenous class of post-translational modifications seems very timely. The number of various glycan moieties that can modify proteins easily surpasses the sum of all other post-translational modifications combined, and the majority of all expressed mammalian proteins (secreted, membrane bound, and intracellular) are glycosylated. Thus, it is essential to be able to characterize these challenging biomolecules to better understand the fundamental roles that glycoconjugates play in nearly all aspects of physiology and pathophysiology.

On the cover: This art highlights from top to bottom the three main topic areas covered by the Special Issue. The first topic area is capturing glycoproteins/glycopeptides (in the purple under shading at the top right) away from the non-glycosylated species (in the green under shading at top left). Then the purple arrow represents introduction into a mass spectrometer for analyses by fragmentation techniques (shown in the yellow under shading region in the middle of the art piece) which is the second major topic area covered in the review. Finally, the third topic area of post-acquisition data analyses (shown in the blue under shading region at the bottom) takes the data generated (yellow arrow) and reassembles the pieces to map sites of modification and determine site-specific microheterogeneity (represented by the moss like structures) on the protein backbone (amino acids displayed as stones across the bottom of the picture).
Art by Julie Newdoll, brushwithscience.com




  • Gerald W. Hart, Lance Wells
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  • Christopher M. West, Daniel Malzl, Alba Hykollari, Iain B.H. Wilson
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  • Nicholas M. Riley, Carolyn R. Bertozzi, Sharon J. Pitteri
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  • Jason C. Maynard, Robert J. Chalkley
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  • Simone Kurz, M. Osman Sheikh, Shan Lu, Lance Wells, Michael Tiemeyer
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  • Ronnie Blazev, Christopher Ashwood, Jodie L. Abrahams, Long H. Chung, Deanne Francis, Pengyi Yang, Kevin I. Watt, Hongwei Qian, Gregory A. Quaife-Ryan, James E. Hudson, Paul Gregorevic, Morten Thaysen-Andersen, Benjamin L. Parker
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  • Rebeca Kawahara, Saulo Recuero, Miguel Srougi, Katia R.M. Leite, Morten Thaysen-Andersen, Giuseppe Palmisano
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  • Colin T. McDowell, Zachary Klamer, Johnathan Hall, Connor A. West, Luke Wisniewski, Thomas W. Powers, Peggi M. Angel, Anand S. Mehta, David N. Lewin, Brian B. Haab, Richard R. Drake
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  • Zhengwei Chen, Qinying Yu, Qing Yu, Jillian Johnson, Richard Shipman, Xiaofang Zhong, Junfeng Huang, Sanjay Asthana, Cynthia Carlsson, Ozioma Okonkwo, Lingjun Li
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  • Marissa Martinez, Santosh Renuse, Simion Kreimer, Robert O’Meally, Peter Natov, Anil K. Madugundu, Raja Sekhar Nirujogi, Raiha Tahir, Robert Cole, Akhilesh Pandey, Natasha E. Zachara
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  • Zilu Ye, Sergey Y. Vakhrushev
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  • Cassandra L. Pegg, Toan K. Phung, Christopher H. Caboche, Suchada Niamsuphap, Marshall Bern, Kate Howell, Benjamin L. Schulz
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  • Lauren E. Pepi, Patience Sanderson, Morgan Stickney, I. Jonathan Amster
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  • Andrea Persson, Mahnaz Nikpour, Egor Vorontsov, Jonas Nilsson, Göran Larson
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  • Weiqian Cao, Mingqi Liu, Siyuan Kong, Mengxi Wu, Yang Zhang, Pengyuan Yang
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  • Daniel G. Delafield, Lingjun Li
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  • Tomislav Čaval, Albert J.R. Heck, Karli R. Reiding
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  • William E. Hackett, Joseph Zaia
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  • Abhishek Roushan, Gary M. Wilson, Doron Kletter, K. Ilker Sen, Wilfred Tang, Yong J. Kil, Eric Carlson, Marshall Bern
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